Inventors:
Joseph Richard Jadamec - Mystic CT
Robert Bauman - Allison Park PA
Carol Patricia Anderson - Mystic CT
Stephen A. Jakubielski - Uncasville CT
Neslie D. Sutton - Uncasville CT
Michael J. Kovacs - Waterford CT
Assignee:
The University of Connecticut - Storrs CT
International Classification:
C12Q 134
Abstract:
The invention is a method for detecting the presence of bacteria in a sample. The method of the invention utilizes the steps of (a) admixing a fluorescent conjugate with a sample to be tested for the presence of bacteria in the sample, wherein a moiety of the fluorescent conjugate is metabolized to release the fluorescent moiety of the fluorescent conjugate; (b) inducing fluorescence of the fluorescent conjugate and the fluorescent moiety of the metabolized fluorescent conjugate at 325-345 nm; (c) simultaneously detecting the fluorescence of the fluorescent conjugate at 370-380 nm and the fluorescent moiety of the metabolized fluorescent conjugate at 445-480 nm; and (d) correlating the detected fluorescence of the fluorescent conjugate and the fluorescent moiety of the metabolized fluorescent conjugate to the presence of bacteria in the sample.