Inventors:
Romas Kazlauskas - Falcon Heights MN, US
Peter Bernhardt - Cambridge MA, US
Assignee:
Regents of the University of Minnesota - Saint Paul MN
International Classification:
C12P 7/64
C12N 15/74
C12N 9/66
C07H 21/04
C12N 9/20
US Classification:
435440, 435218, 435471, 435198, 435 691, 435134, 536 232
Abstract:
The invention provides for methods of modifying n esterase or a lipase enzyme to introduce perhydrolase activity or to increase the already-existing perhydrolase activity. The invention also provides for methods of converting an polypeptide having predominantly esterase and/or lipase catalytic activity to a polypeptide having predominantly perhydrolase activity.