Inventors:
Masaaki Aoyama - Yamagata, JP
Masanobu Shiga - Rockville MD
Assignee:
Yamagata Technopolis Foundation
JEOL Ltd.
International Classification:
C12Q 128
C12Q 126
G01N 3353
G01N 2400
Abstract:
Method for detecting peroxidase or hydrogen peroxide with high sensitivity. Both peroxidase and hydrogen peroxide are prepared such that one of them is overabundant to the other. Phenoxy radicals are produced from a p-substituted phenol compound by the action of peroxidase in the presence of hydrogen peroxide. The free radicals are trapped by a hydroxy amine compound, and stable radical species are produced. Electron spin resonances of the stable radical species are measured.