Augustine A Dinovo

20060027454, Feb 9, 2006

Aug 9, 2005

11/199601

Augustine DiNovo - Charleston SC, US

C07K 1/26

204450000, 530416000

After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number of protein spots visualized by 2DE analysis, and may allow enrichment of proteins normally not detectable by standard 2DE analysis. According to some embodiments of the disclosure, devices, systems, and methods of the disclosure relate to fractionating a proteome on the basis of surface charge, hydrophobicity, isoelectric point and/or size.

20070181427, Aug 9, 2007

Jan 29, 2007

11/668319

Augustine DiNovo - Charleston SC, US

C07K 1/14

204450000, 530412000

After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number of protein spots visualized by 2DE analysis, and may allow enrichment of proteins normally not detectable by standard 2DE analysis. According to some embodiments of the disclosure, devices, systems, and methods of the disclosure relate to fractionating a proteome on the basis of surface charge, hydrophobicity, isoelectric point and/or size.

20080193917, Aug 14, 2008

May 26, 2006

11/914431

David Alexander Schofield - Hollywood SC, US
Augustine Anthony Dinovo - James Island SC, US

C12Q 1/70
C12M 1/00

435 5, 4352871, 4352872

The present disclosure provides a biological detection system and method. One detection system may include a genetically engineered phage that expresses a surface molecule able to bind a target molecule; a bacterium susceptible to infection by the phage; and a detection component able to determine whether the bacterium has been infected by the phage. Infection of a bacterium by a phage may be indicative of phage binding to the target molecule. One method may include placing a sample suspected of containing the target molecule with a binder; adding a genetically engineered phage having reporter genetic material and able to bind the target molecule; washing away unbound phage; releasing phage bound to the target molecule; infecting a bacterium with the released phage; and detecting the presence of any reporter genetic material in the bacterium. Reporter material in the bacterium may correlate with target molecule in the sample.

20110059514, Mar 10, 2011

Jan 7, 2008

12/522233

David Alexander Schofield - Hollywood SC, US
Augustine Anthony Dinovo - James Island SC, US

C12N 1/19
A62D 3/02

43525421, 4352625

The present disclosure relates to yeast biocatalysts and methods of using yeast biocatalysts for detoxifying a biowarfare agent. In some embodiments, a yeast biocatalyst may include a nucleic acid encoding a phage lysin operably linked to a prepro leader sequence, an expression control sequence operably linked to the nucleic acid, and a bioeffective amount (e.g., enough to detoxify) of the phage lysin. A yeast biocatalyst may include an engineered strain of a . A phage lysin may be selected from the group consisting of PlyG and PlyPH. A method of detoxifying a biowarfare agent may include contacting a biowarfare agent with a yeast biocatalyst under conditions that permit detoxification. Conditions that permit detoxification, according to some embodiments, may include conditions that permit expression and secretion of a phage lysin. In some embodiments, conditions that permit detoxification may include conditions that permit germination of a spore. For example, a method of detoxifying a biowarfare agent spore may include contacting the spore with growth media (e.g., complete media or complex media) having alanine.

20120135497, May 31, 2012

Sep 30, 2008

12/241574

David Alexander Schofield - Hollywood SC, US
Augustine Anthony Dinovo - Charleston SC, US

C12N 9/14
C12Q 1/68

435195, 435 61

The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and/or detect the modified form of the chemical agent. The present disclosure also relates, in some embodiments, to variant organophosphorus hydrolase having one or more desirable amino acid substitutions.

20130309746, Nov 21, 2013

Nov 13, 2012

13/675659

Augustine A. DiNovo - Charleston SC, US
Dominic P. DiNovo - Dublin OH, US
David A. Schofield - Hollywood SC, US
Matthew F. Smiechowski - Westerville OH, US
Francis H. Verhoff - Cincinnati OH, US

C12N 11/10

435179, 435177, 435264, 4352625

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.

20130316431, Nov 28, 2013

Nov 13, 2012

13/675576

Augustine A. DiNovo - Charleston SC, US
Dominic P. DiNovo - Dublin OH, US
David A. Schofield - Hollywood SC, US
Matthew F. Smiechowski - Westerville OH, US
Francis H. Verhoff - Cincinnati OH, US

C12N 11/12
C12N 11/02
C12N 11/10

435179, 435178, 435177, 4352625

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked organophosphate-degrading enzyme having a support material which includes a biomass material and/or a polymeric material. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized organophosphate hydrolase enzyme and enzyme materials.