Inventors:
Renfeng Wang - Dublin CA
Lloyd M. Smith - Madison WI
Xinchun E. Tong - Madison WI
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C12Q 170
C07H 2104
C12P 1934
Abstract:
Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.