US Patent:
20070269903, Nov 22, 2007
Inventors:
Cale Halbleib - Middleton WI, US
Kerry Ervin - Middleton WI, US
Mohammed Shekhani - Madison WI, US
Robert Lowery - Brooklyn WI, US
William Checovich - Madison WI, US
Thomas Burke - Madison WI, US
Assignee:
Invitrogen Corporation - Carlsbad CA
International Classification:
G01N 33/58
Abstract:
A method is provided for measuring competitive binding activity of molecules to nuclear receptors comprising mixing a fluorescence-emitting compound that specifically binds to steroid hormone receptors in a solution wherein the fluorescence-emitting compound is in concentration below the dissociation constant and the steroid hormone receptors are at or above the dissociation constant using fluorescence polarization.