Inventors:
Vladimir Poponin - San Francisco CA, US
International Classification:
G01N 21/65
G01J 3/44
US Classification:
436171, 356301, 422 681, 422 8205, 422 8209, 422 8211, 435 6, 436 86, 436 94, 436164, 436518, 436524, 436525
Abstract:
An optical sensor and method for use with a visible-light laser excitation beam and a Raman spectroscopy detector, for detecting the presence chemical groups in an analyte applied to the sensor are disclosed. The sensor includes a substrate, a plasmon resonance mirror formed on a sensor surface of the substrate, a plasmon resonance particle layer disposed over the mirror, and an optically transparent dielectric layer about 2-40 nm thick separating the mirror and particle layer. The particle layer is composed of a periodic array of plasmon resonance particles having (i) a coating effective to binding analyte molecules, (ii) substantially uniform particle sizes and shapes in a selected size range between 50-200 nm (ii) a regular periodic particle-to-particle spacing less than the wavelength of the laser excitation beam. The device is capable of detecting analyte with an amplification factor of up to 10-10, allowing detection of single analyte molecules.