Tuo Li

7126755, Oct 24, 2006

Sep 12, 2003

10/661082

John A. Moon - Wallingford CT, US
Martin A. Putnam - Cheshire CT, US
Alan D. Kersey - South Glastonbury CT, US
Tuo Li - East Lyme CT, US
James Dunphy - South Glastonbury CT, US
F. Kevin Didden - Wallingford CT, US

G02B 5/18

359569, 359 2, 250566

A method and apparatus for labeling an item using diffraction grating-based encoded optical identification elements includes an optical substrate having at least one diffraction grating disposed therein. The grating has one or more colocated pitches Λ which represent a unique identification digital code that is detected when illuminated by incident light. The incident light may be directed transversely from the side of the substrate (or from an end) with a narrow band (single wavelength) or multiple wavelength source, and the code is represented by a spatial distribution of light or a wavelength spectrum, respectively, or a combination thereof. The element can provide a large number of unique codes, e. g. , greater than 67 million codes, and can withstand harsh environments. The encoded element may be used to label any desired item, such as large or small objects, products, solids, powders, liquids, gases, plants, minerals, cells and/or animals, or any combination of or portion of one or more thereof.

7164533, Jan 16, 2007

Jan 22, 2004

10/763995

John Moon - Wallingford CT, US
Alan D. Kersey - South Glastonbury CT, US
Martin A. Putnam - Cheshire CT, US
Tuo Li - East Lyme CT, US

CyVera Corporation - Wallingford CT

G02B 5/18

359569, 4352887, 2505594, 250566

A method and apparatus f or performing an assay process, featuring providing microbeads in a solution; placing the microbeads on an alignment substrate; reading codes of the microbeads and the position thereof on the alignment substrate; reading the fluorescence on each microbead and the position order thereof on the alignment substrate; and determining an assay result based on bead position order and bead code of the earlier reading steps, where the microbead is an encoded particle having a particle substrate; a portion of the substrate being made of a substantially single material and having at least one diffraction grating embedded therein, the grating having a resultant refractive index variation within the single material at a grating location; and the grating providing an optical output signal indicative of a code when illuminated by an incident light signal propagating from outside said substrate, the optical output signal being a result of passive, non-resonant scattering from said grating when illuminated by said incident light signal.

7375890, May 20, 2008

Jun 16, 2006

11/454307

Martin A. Putnam - Cheshire CT, US
John Moon - Wallingford CT, US
Paul S. Szczepanek - Middletown CT, US
Tuo Li - East Lyme CT, US
Anthony Rauseo - Kensington CT, US
Joseph Traynor - Woburn MA, US

Cyvera Corporation - Wallingford CT

G02B 5/18

359566, 359900, 385 37, 385147

A method of manufacturing optical identification elements that includes forming a diffraction grating in a fiber substrate along a longitudinal axis of the substrate. The grating includes a resultant refractive index variation. The method also includes cutting the substrate transversely to form a plurality of optical identification elements that have the grating therein along substantially the entire length of the elements. Each of the elements has substantially the same resultant refractive index variation.

7399643, Jul 15, 2008

Sep 12, 2003

10/661836

John Moon - Wallingford CT, US
Martin A. Putnam - Cheshire CT, US
Tuo Li - East Lyme CT, US
Joseph Traynor - Woburn MA, US

Cyvera Corporation - Wallingford CT

G01N 33/552

436521, 436501

A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (μm) in diameter and between 100 and 500 μm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes.

7441703, Oct 28, 2008

Oct 1, 2004

10/956791

John A. Moon - Wallingford CT, US
David R. Fournier - Northborough MA, US
Martin A. Putnam - Cheshire CT, US
Alan D. Kersey - Glastonbury CT, US
Tuo Li - East Lyme CT, US

Illumina, Inc. - San Diego CA

G06K 7/10

235454, 235435, 235491

An optical reader system for diffraction grating-based encoded microbeads (or bead reader system), comprises a reader box which accepts a bead cell (or cuvette) that holds the microbeads having an embedded code therein. The reader box interfaces along lines with a known computer system The reader box interfaces with a stage position controller and the controller interfaces along a line with the computer system and a manual control device (or joy stick) along a line The reader interrogates the microbeads to determine the embedded code and/or the fluorescence level on the beads. The reader provides information similar to a bead flow cytometer but in a planar format, i. e. , a virtual cytometer.

7659983, Feb 9, 2010

Nov 30, 2006

11/607837

John A. Moon - Wallingford CT, US
Alan D. Kersey - South Glastonbury CT, US
Martin A. Putnam - Cheshire CT, US
Tuo Li - East Lyme CT, US

Electronics and Telecommunications Resarch Institute - Daejon

G01N 21/25

356417, 356244

A method and apparatus for performing an assay process, featuring providing microbeads in a solution, each microbead having a particle substrate with a grating with a superposition of different predetermined regular periodic variations of the index of refraction disposed in the particle along a grating axis and indicative of a code; placing the microbeads on an alignment substrate; reading codes of the microbeads and the position thereof on the alignment substrate; reading the fluorescence on each microbead and the position order thereof on the alignment substrate; and determining an assay result based on bead position order and bead code of the earlier reading steps.

7843567, Nov 30, 2010

Dec 22, 2009

12/644255

John A. Moon - Wallingford CT, US
Alan D. Kersey - South Glastonbury CT, US
Martin A. Putnam - Cheshire CT, US
Tuo Li - East Lyme CT, US

Illumina, Inc. - San Diego CA

G01N 21/25

356417

A method of identifying an analyte. The method includes providing a plurality of microparticles. The microparticles have optically detectable codes extending along bodies of the corresponding microparticle. The microparticles have the chemical probes attached thereto. Each of the chemical probes is associated with a corresponding one of the codes. The method also includes selectively binding target analytes to the chemical probes on the microparticles to produce labeled microparticles and distributing the labeled microparticles to random locations of a substrate. The method also includes determining the codes for the labeled microparticles in the random array and code positions of the codes in the random array. The method further includes detecting the label on the labeled microparticles in the random array and label positions of the labels in the random array. The method also includes using the code positions and the label positions to analyze the target analyte.

7898735, Mar 1, 2011

Mar 21, 2008

12/053242

John A. Moon - Wallingford CT, US
Martin A. Putnam - Cheshire CT, US
Paul S. Szczepanek - Aptos CA, US
Tuo Li - East Lyme CT, US
Anthony Rauseo - Kensington CT, US
Joseph Traynor - N. Berwick ME, US

Illumina, Inc. - San Diego CA

G02B 5/18

359566, 359900, 385 37, 385147

A system for writing an optical code within or on a fiber substrate. The system includes a holding device that has a plurality of supports spaced apart from each other. The fiber substrate is wound about the supports such that the fiber substrate forms at least one flat section extending between adjacent supports. The system also includes at least one light source that is configured to write an optical code within or on the flat section of the fiber substrate.