Terrence J Andreasen

6451607, Sep 17, 2002

Mar 21, 2000

09/423402

Paul J. Lawrence - Campbell CA
Robert Pena - San Jose CA
Terrence J. Andreasen - San Jose CA

Litmus Concepts, Inc. - Santa Clara CA

G01N 3100

436 19, 436 8, 436111, 436163, 436164, 422 61

A control device serving as a source of control reagent for a solid-phase analytical test device is disclosed. The analytical test device analyzes a biological sample for the presence of an analyte such as an enzyme or other chemical species or a particular pH range, and registers the presence or absence of the analyte as a detectable change in an indicator. The control device contains a control reagent that produces the same indicator change and that can be transferred to the analytical test device by a sample implement such as a wet swab. The control reagent is present on the control device as a dry lamina or combination of laminae. A positive control reagent on a control device in accordance with this invention mimics the action of the analyte once it is transferred to the analytical test device, while a negative control reagent on the control device mimics the action of a sample that lacks the analyte.

7041469, May 9, 2006

Oct 10, 2002

10/269917

Paul J. Lawrence - Campbell CA, US
Mark A. Hughes - Morgan Hill CA, US
Aulena Chaudhuri - San Jose CA, US
Terrence J. Andreasen - San Jose CA, US

Quidel Corporation - San Diego CA

C12Q 1/37

435 23, 435 24, 435 34, 435 4, 4352871, 4352873, 4352877

The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at a low pH. Selective assays for trichomoniasis are also obtained by removing particulate matter from a sample of vaginal fluid to extract a fraction devoid of particles greater than a selected size, and where desired, combining the extracted fraction with any of certain specified hydrolase inhibitors, then testing the fraction for enzymatic hydrolase activity. These qualities of trichomoniasis are the basis for a series of diagnostic tests and test devices that produce results that are detectable by visual and other means with a high degree of accuracy.

7291481, Nov 6, 2007

Feb 13, 2006

11/353497

Paul J. Lawrence - Campbell CA, US
Mark A. Hughes - Morgan Hill CA, US
Aulena Chaudhuri - San Jose CA, US
Terrence J. Andreasen - San Jose CA, US

Quidel Corporation - San Diego CA

C12Q 1/02
C12Q 1/34

435 29, 435 18

The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at a low pH. Selective assays for trichomoniasis are also obtained by removing particulate matter from a sample of vaginal fluid to extract a fraction devoid of particles greater than a selected size, and where desired, combining the extracted fraction with any of certain specified hydrolase inhibitors, then testing the fraction for enzymatic hydrolase activity. These qualities of trichomoniasis are the basis for a series of diagnostic tests and test devices that produce results that are detectable by visual and other means with a high degree of accuracy.

20080026417, Jan 31, 2008

Aug 13, 2007

11/838078

Paul Lawrence - Campbell CA, US
Mark Hughes - Morgan Hill CA, US
Aulena Chaudhuri - San Jose CA, US
Terrence Andreasen - San Jose CA, US

Quidel Corporation - San Diego CA

C12Q 1/04
C12Q 1/34

435018000

The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at a low pH. Selective assays for trichomoniasis are also obtained by removing particulate matter from a sample of vaginal fluid to extract a fraction devoid of particles greater than a selected size, and where desired, combining the extracted fraction with any of certain specified hydrolase inhibitors, then testing the fraction for enzymatic hydrolase activity. These qualities of trichomoniasis are the basis for a series of diagnostic tests and test devices that produce results that are detectable by visual and other means with a high degree of accuracy.

55716849, Nov 5, 1996

Jan 17, 1995

8/374487

Paul J. Lawrence - Campbell CA
Terrence J. Andreasen - San Jose CA
David R. Shockey - Cupertino CA

Litmus Concepts, Inc. - Santa Clara CA

C12Q 134
C12N 1108
G01N 2100

435 18

The presence of an enzymatically active hydrolase in a fluid sample is detected by contacting the sample with a solid-phase conjugate which is susceptible to cleavage by the hydrolase, and simultaneously or shortly thereafter, contacting the sample with an indicator which undergoes a detectable change upon the action of a reporter group. The reporter group is part of the conjugate and is liberated from it either partly or entirely by the action of the hydrolase. The indicator is susceptible to action by the reporter group only upon decoupling of the reporter group from the remainder of the conjugate, the decoupling occurring either in part or entirely upon action of the hydrolase. Also provided by this invention are various forms of a dry, self-contained test device which contains the conjugate described above plus the indicator and all other reagents and components necessary to achieve a detectable indication of the presence or absence of a catalytically active hydrolase. Preferred embodiments of the device also contain positive and negative controls.

54160030, May 16, 1995

Apr 14, 1993

8/048536

Paul J. Lawrence - Campbell CA
Terrence J. Andreasen - San Jose CA

Litmus Concepts, Inc. - Santa Clara CA

C12G 134
C12N 1108
G01N 2100

435 18

Methods of assaying the presence of enzymatically active hydrolases (i. e. , hydrolytic enzymes) in a sample or specimen are disclosed. In particular, a method of detecting candidiasis by assaying for the presence of enzymatically active aspartic protease in a sample is provided. In these methods, a sample or specimen is contacted with a solid support. The solid support with which the sample is contacted has a reporter enzyme (i. e. , a signal generating enzyme) immobilized thereon. The reporter enzyme is immobilized on the solid support in a manner such that it is released from the solid support upon action of the enzymatically active hydrolase if the enzymatically active hydrolase is, in fact, present in the sample. The sample after having been contacted with the solid support is combined with an indicator. The indicator is any chemical species which is susceptible to a detectable change, usually a change in color, upon action of the reporter enzyme.

62516213, Jun 26, 2001

Mar 17, 1995

8/406239

Paul J. Lawrence - Campbell CA
Terrence J. Andreasen - San Jose CA

Litmus Concepts, Inc. - Santa Clara CA

C12Q 134

435 18

Methods of assaying for the presence of enzymatically active hydrolases (i. e. , hydrolytic enzymes) in a sample or specimen are disclosed. In particular, a method of detecting candidiasis by assaying for the presence of enzymatically active aspartic protease in a sample is provided. In these methods, a sample or specimen is contacted with a solid support. The solid support with which the sample is contacted has a reporter enzyme (i. e. , a signal generating enzyme) immobilized thereon. The reporter enzyme is immobilized on the solid support in a manner such that it is released from the solid support upon action of the enzymatically active hydrolase if the enzymatically active hydrolase is, in fact, present in the sample. The sample after having been contacted with the solid support is combined with an indicator. The indicator is any chemical species which is susceptible to a detectable change, usually a change in color, upon action of the reporter enzyme.

55852734, Dec 17, 1996

Nov 30, 1994

8/347763

Paul J. Lawrence - Campbell CA
Terrence J. Andreasen - San Jose CA

Litmus Concepts, Inc. - Santa Clara CA

C12M 140
G01N 3122

4352887

A dry, self-contained test device for assaying for the presence of an enzymatically active hydrolase in a sample is disclosed. The test device combines a reporter enzyme immobilized on a solid support, an indicator, and all other reagents and components necessary to achieve a detectable indication of the presence or absence of the enzymatically active hydrolase in the sample. Preferred devices contain positive and negative controls as well.