Tatiana Johnston

15 Humphreys Rd, Barrington, RI 02806

Brown University

2013 to 2014
Teaching Associate
Brown University

Sep 2013 to Nov 2013
Teaching Associate
Brown University

2012 to 2013
Participant
Brown University

2012 to 2013
Development Seminar
Providence College

2012 to 2012
Special Lecturer of Biology
Providence College

2012 to 2012
Adjunct Professor
Seminar

2011 to 2012
Participant
Brown University

2010 to 2012
Instructor (Research)
Brown University

2008 to 2010
Research Associate
Brown University

2005 to 2008
Post Doctoral Research Fellow
Rhode Island Hospital

2003 to 2004
Research Associate
Rhode Island Hospital

2002 to 2003
Research Fellow

University of Szeged
2002
Ph.D. in Molecular and Cell Biology
Research Center of the Hungarian Academy of Sciences
1994
M.S. in General and Molecular Genetics
Taras Shevchenko National University
1993
B.S. in Biology

Skills Cell Culture: various cell-type lines (including primary), retrovirus and lentivirus gene expression delivery systems, transient/stable transfections, electroporation, viability/cytotoxicity assays, cells proliferations assays (CyQuant; Click-IT EdU; BrdU), luciferase assay, immustaining, clonogenic assay Animal: handling of small animals (mice), general surgeries and isolation of peritoneal macrophages DNA: molecular cloning, transformation/electroporation of bacterial cells, DNA isolation from bacterial culture (mini-, midi-, maxi-preps), preparation of genomic DNA from mammalian tissue, cultured cells and bacteria, agarose gel electrophoresis, gel shifts (EMSA) assays, 2D agarose electrophoresis, site-directed mutagenesis, DNA sequencing, Southern hybridization, primer design, PCR, qRT-PCR, cDNA gene arrays RNA: RNA extraction from cultured cells and tissue, RT-PCR, shRNA delivery and expression for gene knockdown Protein: whole cell lysate preparation (cell lysate fractionation), SDS -PAGE, native PAGE, Western blot, protein purification by different affinity purification methods, protein expression in bacterial and mammalian cells, ELISA, antibody arrays, immunoprecipitation and co-immunoprecipitation assays