Shiv Kale Phones & Addresses
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801 Crestwood Dr, Blacksburg, VA 24060
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Fairfax, VA
Publications
Us Patents
Compositions And Methods To Protect Cells By Blocking Entry Of Pathogen Proteins
View pageUS Patent:
20100093601, Apr 15, 2010
Filed:
May 19, 2009
Appl. No.:
12/468470
Inventors:
Brett Tyler - Blacksburg VA, US
Daolong Dou - Nanjing, CN
Shiv Kale - Fairfax VA, US
Biao Gu - Yangling, CN
Daolong Dou - Nanjing, CN
Shiv Kale - Fairfax VA, US
Biao Gu - Yangling, CN
International Classification:
A61K 38/02
A61K 31/661
A61K 31/255
A61K 31/661
A61K 31/255
US Classification:
514 2, 514129, 514517, 514506, 514 54
Abstract:
Pathogenic effector proteins which include one or more RxLR, dEER, Pexel or analogous motifs are blocked from entry into plant or animal cells by binding one or more of the motifs with a blocking compound which prevents binding of phosphoinositides or other polar lipids to the motifs which is a prerequisite for translocation of the pathogenic effector proteins into the plant or animal cell. The blocking compounds can take a variety of forms including synthetic peptides or the hydrophilic head-groups of phosphoinositides, phosphatidic acids, phospholipids, or sphingolipids. Suitable blocking compounds can be identified by assays demonstrating binding to RxLR, dEER, Pexel or analogous motifs. In addition, pathogenic effector proteins can be identified by analyzing whether they contain structural RxLR motifs using hidden markov modeling.
Compositions And Methods To Protect Cells By Blocking Entry Of Pathogen Proteins
View pageUS Patent:
20110212541, Sep 1, 2011
Filed:
Nov 11, 2010
Appl. No.:
12/944345
Inventors:
Brett Tyler - Blacksburg VA, US
Shiv Kale - Fairfax VA, US
Vincenzo Antignani - Blacksburg VA, US
Shiv Kale - Fairfax VA, US
Vincenzo Antignani - Blacksburg VA, US
Assignee:
Virginia Tech Intellectual Properties, Inc. - Blacksburg VA
International Classification:
G01N 33/53
C12N 5/04
C12N 5/07
C12N 5/071
C12N 5/077
C12N 5/078
C12N 5/079
C12N 5/04
C12N 5/07
C12N 5/071
C12N 5/077
C12N 5/078
C12N 5/079
US Classification:
436501, 435410, 435412, 435414, 435415, 435417, 435416, 435325, 435350, 435351, 435366, 435368, 435369, 435370, 435371, 435372
Abstract:
Pathogenic effector proteins include one or more virulence motifs of amino acid consensus sequence BXZ, where B=RK or H; X=any amino acid or is absent; Z=L, M, I, W, Y or F) which bind to target polar lipids on a host (plant or animal) cell as a prerequisite for translocation of the pathogenic effector proteins into the cell. Translocation is prevented by binding blocking compounds to one or more motifs of the effector protein or to the lipid ligands of the host cell. The blocking compounds include synthetic or naturally occurring polypeptides which bind the polar lipids or the motifs, various polar lipids, the hydrophilic head-groups of polar lipids, etc. Suitable blocking compounds can be identified by assays demonstrating binding to the motifs or to the target polar lipids.
Device And Method For Biolistic Transformation Of Cells
View pageUS Patent:
20090011510, Jan 8, 2009
Filed:
Jun 6, 2008
Appl. No.:
12/134906
Inventors:
Shiv D. Kale - Fairfax VA, US
Brett M. Tyler - Blacksburg VA, US
Brett M. Tyler - Blacksburg VA, US
International Classification:
C12N 15/87
C12M 1/00
C12M 1/00
US Classification:
435468, 4352853
Abstract:
The present invention provides a device and method for biolistic bombardment of cells. In a preferred embodiment, the device comprises a hollow tube or cylinder that attaches to a standard biolistic transformation apparatus, and focuses the nucleic acids at cells and tissue sections. In a typical configuration, the device has two barrels that deliver equal or substantially equal amounts of nucleic acids to cells of a tissue, such as a plant leaf.
Compositions And Methods To Protect Cells By Blocking Entry Of Pathogen Proteins
View pageUS Patent:
20140331365, Nov 6, 2014
Filed:
May 13, 2014
Appl. No.:
14/276170
Inventors:
- Blacksburg VA, US
Shiv Kale - Fairfax VA, US
Vincenzo Antignani - Blacksburg VA, US
Shiv Kale - Fairfax VA, US
Vincenzo Antignani - Blacksburg VA, US
Assignee:
VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. - Blacksburg VA
International Classification:
C12N 15/82
US Classification:
800301, 435468
Abstract:
Pathogenic effector proteins include one or more virulence motifs of amino acid consensus sequence BXZ, where B=RK or H; X=any amino acid or is absent; Z=L, M, I, W, or F) which bind to target polar lipids on a host (plant or animal) cell as a prerequisite for translocation of the pathogenic effector proteins into the cell. Translocation is prevented by binding blocking compounds to one or more motifs of the effector protein or to the lipid ligands of the host cell. The blocking compounds include synthetic or naturally occurring polypeptides which bind the polar lipids or the motifs, various polar lipids, the hydrophilic head-groups of polar lipids, etc. Suitable blocking compounds can be identified by assays demonstrating binding to the motifs or to the target polar lipids.