Inventors:
Lonnie O'Neal Ingram - Gainesville FL, US
Shengde Zhou - Gainesville FL, US
Assignee:
University of Florida Research Foundation, Inc. - Gainesville FL
International Classification:
C07H 21/04
C12N 1/12
US Classification:
4352521, 435 691, 435183, 4353201, 435325, 536 232, 536 234, 536 235, 536 236, 536 237, 536 241
Abstract:
The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.