Shakeel M Thakurdas

Houston, TX

SOUTHERN CALIFORNIA RETINA CENTER, CA

Dec 2010 to Oct 2011
Practice Manager
UNIVERSITY OF TEXAS MD ANDERSON CANCER
Center, TX
May 2004 to Jul 2009
Research Assistant II (Mast cell exocytic mechanisms)
BAYLOR COLLEGE OF MEDICINE
Houston, TX
Jul 2003 to Apr 2004
Research Assistant II
THE AGA KHAN UNIVERSITY, DEPT OF MICROBIOLOGY, KARACHI, PAKISTAN

Apr 2002 to Jun 2003
Instructor
THE AGA KHAN UNIVERSITY, DEPT OF MICROBIOLOGY, KARACHI, PAKISTAN

Jul 1999 to Mar 2002
Research Medical Officer

The Aga Khan University Medical College Karachi
London
Sep 1991 to Mar 1999
MBBS in CLINICAL
University of London
Jun 1987 to Jun 1990
Baylor College of Medicine
Ben Taub General Hospital
Patrick's High School

HONORS & AWARDS Cyrus Award Finalist for Outstanding Clinical Research, MD Anderson, UT - 2009 Performance Recognition Award, MD Anderson, University of Texas - 2006 Performance Recognition Award, MD Anderson, University of Texas 2005 PRIVATE PRACTICE SKILLS Starting-up and running a private medical practice, knowledge of marketing, management, medical billing & collections and strategic &business planning TECHNICAL EXPERIENCE Molecular genetics Designing & creating DNA constructs to alter the genes expression in mice, Maintenance, screening and experimentation on genetically engineered mice, Genomic DNA isolation; subcloning; Southern blot and direct gel hybridization; computer analysis of genetic data Immunology Antisera generation and monoclonal antibody isolation; isotopic, fluorescent, and biotin labeling of cellular proteins and antibodies; immunoprecipitation; ELISA; Histological preparations, staining and microscopy, FACS (Fluorescent Antibody Cell Sorting) analysis Biochemistry DNA and RNA extraction and purification, Agarose gel electrophoresis, DNA cloning, utilizing polymerase chain reaction (PCR) technology, aseptic techniques, and biological assays, RT-PCR, DNA sequencing, Southern blot, DNA cloning, Recombineering; flow cytometry; Protein extraction and detection by gel filtration & Western blot, protein quantification by ELISA; Spectrophotometric enzyme assays Tissue Culture Cell and tissue harvesting from mice, mast cell cultures, Functional assays such as mast cell cytokine release upon stimulation, cell separation, including Percoll density gradient and centrifugal elutriation; Mouse dissections, peritoneal lavage, broncho-alveolar lavage, intradermal injection Instrumental Expertise Spectrophotometer, Spectrofluorimeter, Microscopes, Confocal Microscopy, Electrophoresis and Blotting systems, FACS, real-time PCR, electroporator TEACHING SKILLS Educated MBBS and PhD student as an Instructor of Microbiology and demonstrated scholarship through research/publications SYSTEMS & IT SKILLS Working experience of Microsoft Office (Excel, Word, Power Point and Outlook) Advance level User of Adobe Photoshop Working experience of software & programs used in genetics and molecular biology including GeneRunner (genetics software), Primer3 (Program for designing PCR primers), Gel Documentation software, etc. Working experience of Epi Info statistical analysis program and Reference Manager - Software tool for publishing and managing bibliographies on the Windows and Macintosh