Sergei A Kozyavkin

20060068386, Mar 30, 2006

Mar 4, 2003

10/506454

Alexei Slesarev - Boyds MD, US
Andrei Malykh - Germantown MD, US
Andrey Pavlov - Gaithersburg MD, US
Nadezhda Pavlova - Gaithersburg MD, US
Sergei Kozyavkin - Germantown MD, US

C12Q 1/68
C07H 21/04
C12P 21/06
C07K 16/12
C07K 14/195

435006000, 435069100, 435252300, 435320100, 530350000, 536023700, 530388400

We have determined the complete 1,694,969 nucleotide sequence of the GC-rich genome of using a novel approach. It is based on unlinking genomic DNA with the ThermoFidelase version of topoisomerase V and cycle sequencing directed by 2′-modified oligonucleotides (Fimers). 3.3× sequencing redundancy was sufficient to assemble the genome with

20060234227, Oct 19, 2006

May 14, 2003

10/514342

Alexei Slesarev - Boyds MD, US
Andrey Pavlo - Gaithersburg MD, US
Sergei Kozyavkin - Germantown MD, US
Nadezhda Pavlova - Gaithersburg MD, US

C12Q 1/68
C12P 19/34
C12N 9/22

435006000, 435199000, 435091200

The utility of Topo V's HhH motifs for modulating DNA binding properties of a DNA processing enzyme, such as Taq DNA polymerase or PfuDNA polymerase, is demonstrated. In one embodiment, an amino acid residue comprising one or more HhH domains derived from Topoisomerase V, linked to either the NH-terminus or COOH-terminus of a Taq polymerase fragment significantly broadens the processivity and/or salt concentration range of polymerase activity. The specific activities of the chimeric polymerases are not affected by added HhH motifs. Depending on the type of the construct, the thermal stability of chimeric polymerases increases or remains the same as that of Taq DNA polymerase or its Stoffel fragment. This invention further provides a method of increasing the salt tolerance of Taq polymerases. The methods of this invention may be applied to all pol A-type DNA polymerases, as well as to other DNA processing enzymes.

6548251, Apr 15, 2003

Sep 5, 2000

09/655804

Sergei A. Kozyavkin - Germantown MD
Andrei G. Malykh - Germantown MD
Nikolai N. Polouchine - Montgomery Village MD
Alexei I. Slesarev - Germantown MD

Fidelity Systems, Inc. - Gaithersburg MD

C12Q 168

435 6, 435 911, 435 912, 514 44, 536 231, 536 242, 536 243, 536 2433, 536 245

A method of inhibiting at least one molecular process in a sample, comprising administering to the sample an oligonucleotide or polynucleotide containing at least one monomeric unit having formula (I): wherein A is an organic moiety, n is at least 1, and each X is independently selected from the group consisting of âNRCOCONu, âNHCOCR CR CONu, âNHCOCR CRCONu, and âNHCOSSCONu, wherein each R independently represents H or a substituted or unsubstituted alkyl group, and Nu represents a nucleophile, or a salt of the compound.