US Patent:
20100035292, Feb 11, 2010
Inventors:
Andre Levhenko - Columbia MD, US
Alexander Groisman - San Diego CA, US
Saurabh Paliwal - Baltimore MD, US
Assignee:
The Johns Hopkins University - Baltimore MD
International Classification:
C12Q 1/02
C12M 1/00
Abstract:
The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.
