Ryan Anthony Eberwine

2612 Folsom St, Philadelphia, PA 19130

Pharmaceuticals

The Janssen Pharmaceutical Companies of Johnson & Johnson
Senior Scientist
Drexel University College of Medicine May 2013 - Apr 2015
Post-Doctoral Fellow
Drexel University College of Medicine 2008 - 2013
Graduate Student Researcher
Upmc 2005 - 2008
Research Associate

Drexel University 2008 - 2013
Doctorates, Doctor of Philosophy, Molecular Biology, Genetics, Philosophy University of Pittsburgh 2003 - 2007
Bachelors, Bachelor of Science, Biology Wissahickon Senior High School

Multi Color Flow Cytometry
T Cell Functional Assays
Immunoassays
Primary Cell Isolation
Assay Development
Immunofluorescence
Molecular Biology
Cell Culture
Pcr
Cell Biology
Genetics
Real Time Pcr
Pcr Primer Design
Molecular Genetics
Bioinformatics
Recombinant Dna Technology
Immunology
Sequence Analysis
Western Blotting
Immunohistochemistry
Tissue Culture
Microscopy
Fluorescence Microscopy
Life Sciences
Molecular Cloning
Protein Purification
Rna Isolation
Dna Sequencing
Dna Isolation
Elisa
Affinity Purification
Plasmid Engineering
Transfection
Transduction
Rodent Handling
Facs Analysis
Reporter Gene Assays
Bacterial Transformation
Statistical Software
Cell Sorting
Cell Based Assays
Immunocytochemistry

Politics
Science and Technology
Education
Health

Italian

Drexel University College of Medicine

2013 to 2000
Post-Doctoral Fellow
Drexel University College of Medicine
Philadelphia, PA
2008 to 2013
Graduate Student Researcher
University of Pittsburgh Medical Center
Pittsburgh, PA
2006 to 2008
Research Technician
University of Pittsburgh Medical Center
Pittsburgh, PA
2005 to 2006
Research Technician

Drexel University College of Medicine
Philadelphia, PA
2013
Ph.D. in Molecular & Cellular Biology and Genetics
University of Pittsburgh
Pittsburgh, PA
2007
BS in Biological Sciences

Molecular Genetics, RNA/DNA preparation and handling, Plasmid DNA construction and preparation, Cloning and bacterial transformation, Luminescent reporter assays (promoter, miRNA knock-down), PCR, Quantitative real-time PCR, 5'RACE (Rapid Amplification of cDNA Ends), Gel electrophoresis, Sequencing (sanger, high-throughput/454), Bioinfomatic analysis of genetic sequences, Genotyping, sequencing, microsatellite/marker analysis, allele-specific PCR for SNP detection, Immunology, Immunofluorescence & imaging by DIC and confocal microscopy, ELISA, Western blot, FACS, Affinity purification of antibodies, Tissue Culture (Mammalian adherent and suspension culture), Isolation of cells from primary mammalian tissue, Cell separation by density gradient, Production of retrovirus, Retroviral transduction of cell lines, Plasmid transfection, Rat and mouse handling and colony maintenance, I.P. injection, Small rodent anesthesia, Genetic manipulation/gene knock-out with zinc finger nuclease technology, Scientific writing: manuscript preparation, grant development, editing Statistical analysis: GraphPad/Prism