Robert W Hess

6448065, Sep 10, 2002

Mar 19, 2001

09/812262

Robert A. Hess - Cambridge MA

BBI Bioseq. Inc. - West Bridgewater MA

C12M 134

4352872, 435 6, 435 71, 435 911, 435 912, 4352831, 4352852, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

The invention is based on the discovery that the sequence of monomers in a polymeric biomolecule can be determined in a self-contained, high pressure reaction and detection apparatus, without the need for fluid flow into or out from the apparatus. The pressure is used to control the activity of enzymes that digest the polymeric biomolecule to yield the individual monomers in the sequence in which they existed in the polymer. High pressures modulate enzyme kinetics by reversibly inhibiting those enzymatic processes which result in a higher average activation volume, when compared to the ground state, and reversibly accelerating those processes which have lower activation volumes than the ground state. Modulating the pressure allows the experimenter to precisely control the activity of the enzyme. Conditions can be found, for example, where the enzyme removes only one monomer (e. g.

6696019, Feb 24, 2004

Aug 7, 2001

09/924266

David W. Bradley - Manchester NH
Robert A. Hess - Arlington MA

BBI BioSeq, Inc. - West Bridgewater MA

A61L 202

422 39, 435236

The invention is based on the discovery that biological and non-biological materials can be sterilized, decontaminated, or disinfected by repeatedly cycling between relatively high and low pressures. Pressure cycling can be carried out at low, ambient, or elevated temperatures (e. g. , from about -40Â C. to about 95Â C. , or intermediate ranges). New methods based on this discovery can have applications in, for example, the preparation of vaccines, the sterilization of blood plasma or serum, plant, animal, and human tissue, sputum, urine, feces, water, and ascites, the decontamination of military devices, food and beverage production, and the disinfection of medical equipment. The new methods can also be incorporated into production processes or research procedures.

6716629, Apr 6, 2004

Oct 10, 2001

09/975496

Robert A. Hess - Arlington MA
John Linton - Lincoln MA
Tanya S. Kanigan - Cambridge MA
Colin Brenan - Marblehead MA
Can Ozbal - Cambridge MA

BioTrove, Inc. - Cambridge MA

C12N 502

435420, 435410, 4352831, 4352861, 4352882

The invention features methods of making devices, or âplatensâ, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

6753169, Jun 22, 2004

Jul 9, 2001

09/901297

Robert A. Hess - Brighton MA
David J. Green - Fitzwilliam NH

BBI, BioSeq, Inc. - West Bridgewater MA

C12P 1934

435 912, 435 6, 435 71, 435 911, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

A method of hybridizing a first nucleic acid to a second nucleic acid at least partially complementary to the first nucleic acid by (1) providing a sample vessel and pressure controller for the vessel; and (2) contacting the first and second nucleic acids within the vessel at a pressure above ambient pressure that is effective to enhance hybridization of the first and second nucleic acids.

7626017, Dec 1, 2009

Aug 16, 2004

10/918878

Robert A. Hess - Arlington MA, US
Feng Tao - Montgomery Village MD, US

Pressure Biosciences, Inc. - South Easton MA

C07H 1/06
C07H 1/08

536127, 536 254, 536 2541, 536 2542, 536 2712, 536124, 435 13, 435 72, 435 721, 435 722, 435 731, 435 732

Methods for cell lysis and purification of biological materials, involving subjecting a sample to high pressure. Also featured is an apparatus for practicing the methods.

7682565, Mar 23, 2010

Dec 22, 2003

10/744580

John Linton - Lincoln MA, US
Karl Yoder - Stoneham MA, US
Robert Hess - Arlington MA, US
Leila Hasan - Boston MA, US
Robert Ellis - Burlington MA, US
Tanya S. Kanigan - Cambridge MA, US
Kristine Friesen - Cambridge MA, US
Arrin Katz - Somerville MA, US
Colin Brenan - Marblehead MA, US
Tom Morrison - Winchester MA, US
Javier Garcia - Lexington MA, US

BioTrove, Inc. - Woburn MA

G01N 31/22
G01N 21/01
G01N 21/77

422 681, 422102, 422104, 422 58, 422 8205, 422 8209, 436164, 436165, 436171

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

8105554, Jan 31, 2012

Mar 11, 2005

11/078196

Tanya S. Kanigan - Lexington MA, US
Steve Smith - Arlington MA, US
John Linton - Lincoln MA, US
Robert Hess - Arlington MA, US
Karl Yoder - Stoneham MA, US
Colin Brennan - Marblehead MA, US

Life Technologies Corporation - Carlsbad CA

B01L 3/00

422504, 422 67, 422400, 506 33, 137 67

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

8545772, Oct 1, 2013

Dec 23, 2011

13/336902

Tanya Kanigan - Winchester MA, US
Steve Smith - Arlington MA, US
John Linton - Lincoln MA, US
Robert Hess - Arlington MA, US
Karl Yoder - Stoneham MA, US
Colin Brennan - Marblehead MA, US

Life Technologies Corporation - Carlsbad CA

G01N 1/00
B01L 3/00

422503, 422 681, 422277, 422278, 422504, 422552, 436176, 506 16, 506 39, 137 1, 137803

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.