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Riccardo Barrile

from Crescent Springs, KY
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Connected to:
Anthony C Barrile, 78Brittany C Barrile, 34Barry J Barrilleaux, 49Kay M Barrilleaux, 71Lauren M Barrilleaux, 35Luke J Barrilleaux, 45Trent C Barrilleaux, 36Adam Barringer, 58Alicia K Barrington, 43Allison L Barringer, 50

Riccardo Barrile Phones & Addresses

  • Crescent Springs, KY
  • Boston, MA

Work

Company: University of cincinnati Jan 2020 Position: Assistant professor

Education

Degree: Masters School / High School: Università Degli Studi Di Palermo 2005 to 2010 Specialities: Molecular Biology

Skills

Molecular Biology • Cell Biology • Cell Culture • Biotechnology • Vaccines • Molecular Cloning • Lifesciences • Cell

Industries

Biotechnology

Resumes

Resumes

Riccardo Barrile Photo 1

Assistant Professor

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Location:
Boston, MA
Industry:
Biotechnology
Work:
University of Cincinnati
Assistant Professor
Emulate, Inc.
Associate Director
Emulate, Inc.
Principal Investigator
Cedars-Sinai Medical Center Sep 2015 - Mar 2017
Postdoctoral Researcher
Wyss Institute For Biologically Inspired Engineering May 2014 - Aug 2015
Post Doctoral Fellow
Education:
Università Degli Studi Di Palermo 2005 - 2010
Masters, Molecular Biology Università Degli Studi Di Siena
Doctorates, Doctor of Philosophy, Molecular Biology, Philosophy
Skills:
Molecular Biology
Cell Biology
Cell Culture
Biotechnology
Vaccines
Molecular Cloning
Lifesciences
Cell

Publications

Us Patents

Stem Cell-Based Lung-On-Chip Models

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US Patent:
20210062129, Mar 4, 2021
Filed:
Aug 3, 2020
Appl. No.:
16/983850
Inventors:
- Boston MA, US
Riccardo Barrile - Boston MA, US
David Conegliano - Boston MA, US
Remi Villenave - Boston MA, US
Carolina Carolina - Wastwood MA, US
Justin Nguyen - Medford MA, US
Antonio Varone - West Roxbury MA, US
Catherine Karalis - Brookline MA, US
Geraldine Hamilton - Boston MA, US
International Classification:
C12M 3/06
C12N 5/071
C12N 5/074
Abstract:
An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation. Additionally, these microfluidic “stem cell-based Lung-on-Chip” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for diseases, infections and for reducing inflammation effecting lung alveolar and/or epithelial regions. Further, fluidic devices are provided seeded with primary alveolar cells for use in providing a functional Type II and Type I cell layer, wherein Type II cells express and secrete surfactants, such as Surfactant B (Surf B; SP-B) and Surfactant C (Surf C; SP-C), which were detectable at the protein level by antibody staining in Type II cells. A number of uses are contemplated for the devices and cells, including but not limited to, for use under inflammatory conditions, in drug development and testing, and for individualized (personalized) medicine. Moreover, an ALI-M was developed for supporting multiple cell types in co-cultures with functional Type II and Type I cells.

In Vitro Epithelial Models Comprising Lamina Propria-Derived Cells

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US Patent:
20210031197, Feb 4, 2021
Filed:
Sep 17, 2020
Appl. No.:
17/024221
Inventors:
- Boston MA, US
Riccardo Barrile - Boston MA, US
Geraldine Hamilton - Boston MA, US
Catherine Karalis - Brookline MA, US
Daniel Levner - Brookline MA, US
Carolina Lucchesi - Westwood MA, US
Antonio Varone - West Roxbury MA, US
Remi Villenave - Boston MA, US
International Classification:
B01L 3/00
C12M 1/00
C12M 3/06
C12M 1/42
G01N 33/50
Abstract:
An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.

Method For Assessing A Compound Interacting With A Target On Epithelial Cells

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US Patent:
20210003559, Jan 7, 2021
Filed:
Jun 25, 2020
Appl. No.:
16/912391
Inventors:
- Little Falls NJ, US
- Basel, CH
- Boston MA, US
Annie Moisan - Basel, CH
Nikolche Gjorevski - Basel, CH
Jordan S. Kerns - Boston MA, US
Geraldine A. Hamilton - Boston MA, US
Catherine Karalis - Boston MA, US
Heather Grant - Boston MA, US
Riccardo Barrile - Boston MA, US
Debora Barreiros Petropolis - Boston MA, US
Chaitra Belgur - Boston MA, US
International Classification:
G01N 33/50
Abstract:
Disclosed herein is a method for assessing a compound interacting with a target on polarized epithelial cells. The method comprising the steps of providing an organ chip comprising a main channel and polarized epithelial cells, wherein the main channel is divided into an apical channel and a basal channel separated by the polarized epithelial cells, wherein the apical side of the polarized epithelial cells is directed towards the apical channel and the basolateral side of the polarized epithelial cells is directed towards the basal channel. Determining the localization and optionally the expression level of the target on the polarized epithelial cells. Administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the basal channel, when the target is localized on the basolateral side of the epithelial cells or administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the apical channel, when the target is localized on the apical side of the epithelial cells. Measuring a parameter of the administration of the compound and the peripheral blood mononuclear cells.

Rheologically Biomimetic Fluid Surrogate

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US Patent:
20200332240, Oct 22, 2020
Filed:
Mar 16, 2020
Appl. No.:
16/820530
Inventors:
- Boston MA, US
Magdalena Kasendra - Boston MA, US
Carolina Lucchesi - Westwood MA, US
S. Jordan Kerns - Reading MA, US
Riccardo Barrile - Boston MA, US
Sonalee Barthakur - Boston MA, US
International Classification:
C12M 3/06
B01L 3/00
C12M 1/00
C12M 1/12
C12N 5/071
G01N 33/50
Abstract:
The present invention contemplates compositions, devices and methods of simulating biological fluids in a fluidic device, including but not limited to a microfluidic chip. In one embodiment, fluid comprising a colloid under flow in a microfluidic chip has a fluid density or viscosity similar to a bodily fluid, e.g. blood, lymph, lung fluid, or the like. In one embodiment, a fluid is provided as a Theologically biomimetic blood surrogate or substitute for simulating physiological shear stress and cell dynamics in fluidic device, including but not limited to immune cells.

Methods, Systems, And Compositions For Determining Blood Clot Formation, And Uses Thereof

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US Patent:
20200292531, Sep 17, 2020
Filed:
Apr 29, 2020
Appl. No.:
15/929387
Inventors:
- Cambridge MA, US
- Boston MA, US
Andries D. van der Meer - Enschede, NL
Alan David Michelson - Boston MA, US
Riccardo Barrile - Boston MA, US
International Classification:
G01N 33/50
B01L 3/00
G01N 33/86
Abstract:
A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

In Vitro Epithelial Models Comprising Lamina Propria-Derived Cells

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US Patent:
20180185844, Jul 5, 2018
Filed:
Nov 21, 2017
Appl. No.:
15/819435
Inventors:
- Boston MA, US
Riccardo Barrile - Boston MA, US
Geraldine Hamilton - Boston MA, US
Catherine Karalis - Brookline MA, US
Daniel Levner - Brookline MA, US
Carolina Lucchesi - Westwood MA, US
Antonio Varone - West Roxbury MA, US
Remi Villenave - Boston MA, US
International Classification:
B01L 3/00
G01N 33/50
Abstract:
An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.

Additive Channels

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US Patent:
20180015455, Jan 18, 2018
Filed:
Jul 12, 2017
Appl. No.:
15/648049
Inventors:
- Boston MA, US
- Horsham PA, US
Norman Wen - West Roxbury MA, US
Jacob Fraser - Somerville MA, US
Justin Nguyen - Medford MA, US
Riccardo Barrile - Boston MA, US
Geraldine Hamilton - Boston MA, US
Catherine Karalis - Brookline MA, US
Hyoung Shin Park - Newton MA, US
Antonio Varone - West Roxbury MA, US
Andries Van der Meer - Enchede, NL
Monica Otieno - Robbinville NJ, US
David Conegliano - Boston MA, US
International Classification:
B01L 3/00
C12M 1/00
Abstract:
Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

Additive Channels

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US Patent:
20180015462, Jan 18, 2018
Filed:
Jul 12, 2017
Appl. No.:
15/648085
Inventors:
- Boston MA, US
- Horsham PA, US
Norman Wen - West Roxbury MA, US
Jacob Fraser - Somerville MA, US
Justin Nguyen - Medford MA, US
Riccardo Barrile - Boston MA, US
Geraldine Hamilton - Boston MA, US
Catherine Karalis - Brookline MA, US
Hyoung Shin Park - Newton MA, US
Antonio Varone - West Roxbury MA, US
Andries Van der Meer - Enchede, NL
Monica Otieno - Robbinville NJ, US
David Conegliano - Boston MA, US
International Classification:
B01L 3/00
G01N 33/86
Abstract:
Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.
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Riccardo Barrile from Crescent Springs, KY Get Report