Inventors:
Rhonda Lunt Sundberg - Siler City NC, US
Robert Leonard Hopfer - Moscow PA, US
Assignee:
Wyeth Holdings Corporation - Madison NJ
International Classification:
B01D 15/08
US Classification:
210635, 210656, 2101982, 530413, 536 231, 536 237
Abstract:
The present invention relates to the purification of polypeptides and the removal of endotoxin via immobilized metal affinity chromatography (IMAC). More specifically, the invention relates to methods for removing bacterial endotoxin in a protein solution. In specific embodiments, the invention relates to the elimination of endotoxin from outer membrane proteins.