Rebecca W Vanderslice

20020103370, Aug 1, 2002

Nov 13, 2001

09/986803

Daniel Doherty - Boulder CO, US
Donna Ferber - Louisville CO, US
John Marrelli - Houston TX, US
Rebecca Vanderslice - Boulder CO, US
Randal Hassler - Lafayette CO, US

C07H013/02

536/119000, 536/123000

Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta-[1,4] configuration. The inner D-mannose moieties are linked in an alpha-[1,3] configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta-[1,2] configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta-[1,4] configuration. Processes for preparing the polysaccharide polymers are also provided.

47134496, Dec 15, 1987

Aug 6, 1985

6/762878

Rebecca W. Vanderslice - Boulder CO

Getty Scientific Development Company - Houston TX

C08B 3700
C08B 3718

536123

A polysaccharide polymer is disclosed which is a better viscosifier of water than xanthan gum. The polysaccharide polymer and its non-acetylated form, are comprised of glucose and mannose moieties in a ratio of about 2:1. The invention also discloses Xanthomonas mutants which produce the polysaccharide polymer but which do not produce xanthan gum. Methods of preparing the polysaccharide polymers and of their use are also described.

63166143, Nov 13, 2001

Jun 7, 1995

8/475823

Daniel H. Doherty - Boulder CO
Donna M. Ferber - Boulder CO
John D. Marrelli - Houston TX
Rebecca W. Vanderslice - Boulder CO
Randal A. Hassler - Lafayette CO

CP Kelco U.S., Inc. - Wilmington DE

C08B 3700
C07H 100
C07H 1302
C12P 1906

536114

Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta-[1,4] configuration. The inner D-mannose moieties are linked in an alpha-[1,3] configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta-[1,2] configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta-[1,4] configuration. Processes for preparing the polysaccharide polymers are also provided.

58612548, Jan 19, 1999

Jan 31, 1997

8/792075

Daniel J. Schneider - Broomfield CO
Rebecca Vanderslice - Boulder CO
Larry Gold - Boulder CO

NeXstar Pharmaceuticals, Inc. - Boulder CO

C12P 1934
C12Q 168
C07H 2102
G01N 2101

435 6

Described herein are methods for improved partitioning between high and low affinity nucleic acid ligands identified through the SELEX method, termed Flow Cell SELEX. The Flow Cell SELEX method achieves partitioning between high and low affinity nucleic acid ligands using surface plasmon resonance technology. The method of the present invention presents a new and powerful approach to select nucleic acid ligands by providing a partitioning method which 1) enables a significant increase in the efficiency of partitioning from traditional partitioning methods used in SELEX, 2) allows for real time monitoring of the partitioning of the high affinity ligands from the low affinity ligands 3) allows for the ability to select for a nucleic acid ligand having specific kinetic properties, 4) does not rely on radiolabeling or other means of tagging for detection, and 5) allows for use of smaller amounts of target than in traditional methods of SELEX.

55147917, May 7, 1996

Apr 25, 1994

8/232416

Daniel H. Doherty - Boulder CO
Donna M. Ferber - Louisville CO
John D. Marrelli - Houston TX
Rebecca W. Vanderslice - Boulder CO
Randal A. Hassler - Lafayette CO

Getty Scientific Development Company - Houston TX

C08B 3700
C12P 1906

536114

Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta- 1,4! configuration. The inner D-mannose moieties are linked in an alpha- 1,3! configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta- 1,2! configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta- 1,4! configuration. Processes for preparing the polysaccharide polymers are also provided.

61438669, Nov 7, 2000

Jan 19, 1995

8/375242

Michael T. Brewer - Boulder CO
Karin K. Hale - Boulder CO
Michael W. King - Terre Haute IN
Tadahiko Kohno - Louisville CO
Charles Squires - Boulder CO
Robert C. Thompson - Boulder CO
Rebecca W. Vanderslice - Boulder CO
James Vannice - Boulder CO

Amgen, Inc. - Thousand Oaks CA

C07K 14705
C12N 100

530350

At least two substantially purified tumor necrosis factor (TNF) inhibitors are disclosed which are glycoproteins that are active against TNF. The isolation of 3O kDa and 40 KDa TNF inhibitor from urine is disclosed. The deglycosylated form of the 3O kDa TNF inhibitor and 40 kDa TNF inhibitor are described as being active against TNF. The 40 kDa TNF inhibitor is active against both TNF alpha a TNF beta. The amino acid sequence of the 30 kDa TNF inhibitor and the 40 kDa TNF inhibitor are disclosed. Methods for isolating the TNF inhibitors from human U937 cell medium and producing the proteins by recombinant-DNA methods are also described.

55590152, Sep 24, 1996

Dec 2, 1994

8/352216

Michael A. Capage - Boulder CO
Daniel H. Doherty - Boulder CO
Michael Betlach - Boulder CO
Rebecca W. Vanderslice - Boulder CO

Getty Scientific Development Company - Houston TX

C12P 1906
C12N 121
C12N 1570
C12N 1574

435104

Methods for the recombinant-DNA mediated production of xanthan gum and gum variants structurally related to xanthan are disclosed. The methods in part involve the synthesis of these polysaccharides in anaerobic and/or denitrifying hosts. In particular, plasmids pX209 and pRK290-H366 are disclosed which contain the genes, isolated from X. campestris, encoding Transferase I, Transferases II, Transferase III, Transferase IV, Transferase V, Ketalase, Acetylase and Polymerase. These plasmids have been deposited in the American Type Culture Collection under Accession Nos. 67051 and 67049, respectively.

58244727, Oct 20, 1998

Jun 6, 1995

8/467145

Michael R. Betlach - Boulder CO
Daniel H. Doherty - Boulder CO
Rebecca W. Vanderslice - Boulder CO

Monsanto Company - St. Louis MO

C12Q 168
C12P 2100
C12P 1906
C12N 121

435 6

A recombinant-DNA mediated method for the synthesis of sugar nucleotides is disclosed. This method utilizes portable DNA sequences capable of directing the microbial synthesis of various enzymes that catalyze the synthesis of sugar nucleotides, including UDP-glucose, UDP-glucuronic acid and GDP-mannose. The sugar moieties of these sugar nucleotides may subsequently be incorporated into industrially-useful polysaccharides such as xanthan gum. It has been found that vectors containing the portable DNA sequences described herein are capable both of causing sugar nucleotide production in microorganisms previously incapable of such synthesis and of causing increased sugar nucleotide production in organisms capable of synthesizing small quantities of these compounds. In particular, plasmids pAS7, pAS9 and pTS13 are disclosed. These plasmids are capable of directing sugar nucleotide synthesis in various hosts, including Xanthomonas sp.