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Rashmi Rohit Kshirsagar

from Ashland, MA
Age ~48
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Also known as:
Rashmi R Kshirsagar, Rohit Kshirsagar, Rashmi Hemant Korke, Rashmi Hermant Korke, Rashmi H Korke, Rash Kshirsagar, Kshirsagar Rash
Phone and address:
4 Wadsworth Rd, Ashland, MA 01721
(508) 309-6425
Connected to:
Anurag Kshirsagar, 39Chaitanya Kshirsagar, 42Omkar U Kshirsagar, 48Prashant A Kshirsagar, 66Pravin Kshirsagar, 45Rajshree Kshirsagar, 51Rashmi R Kshirsagar, 60Sonal S Kshirsagar, 39Sumant S Kshirsagar, 40Suyash B Kshirsagar, 41

Rashmi Kshirsagar Phones & Addresses

  • 4 Wadsworth Rd, Ashland, MA 01721 (508) 309-6425
  • Somerville, MA
  • Cambridge, MA
  • Minneapolis, MN

Work

Position: Clerical/White Collar

Education

Degree: High school graduate or higher

Publications

Us Patents

Methods Of Preventing And Removing Trisulfide Bonds

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US Patent:
20120264916, Oct 18, 2012
Filed:
Oct 1, 2010
Appl. No.:
13/499858
Inventors:
David Evans - Melrose MA, US
R. Blake Pepinsky - Arlington MA, US
Dingyi Wen - Waltham MA, US
Rashmi Rohit Kshirsagar - Ashland MA, US
Karin Lucas - Encinitas CA, US
Assignee:
Biogen Idec MA Inc. - Cambridge MA
International Classification:
C07K 1/113
C12P 21/00
C07K 16/00
US Classification:
5303871, 530408, 435 711, 435 703
Abstract:
The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Mammalian Cell Culture-Produced Neublastin Antibodies

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US Patent:
20190194602, Jun 27, 2019
Filed:
Dec 21, 2018
Appl. No.:
16/230216
Inventors:
- Providence RI, US
Kyle McElearney - Medford MA, US
Terrence Michael Dobrowsky - Cambridge MA, US
Rashmi Rohit Kshirsagar - Ashland MA, US
International Classification:
C12N 5/00
C12P 21/00
C07K 14/755
Abstract:
The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Medium Supplements For Improved Process Performance

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US Patent:
20170029859, Feb 2, 2017
Filed:
Oct 12, 2016
Appl. No.:
15/291257
Inventors:
- Cambridge MA, US
Kyle McElearney - Medford MA, US
Terrence Michael Dobrowsky - Cambridge MA, US
Rashmi Rohit Kshirsagar - Ashland MA, US
International Classification:
C12P 21/00
C07K 14/755
C12N 5/00
Abstract:
The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

Use Of Perfusion Seed Cultures To Improve Biopharmaceutical Fed-Batch Production Capacity And Product Quality

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US Patent:
20160289633, Oct 6, 2016
Filed:
Dec 19, 2014
Appl. No.:
15/038698
Inventors:
- Cambridge MA, US
Jiuyi LU - Ipswich MA, US
Yao-Ming HUANG - Cary NC, US
Hang YUAN - Lexington MA, US
Rashmi KSHIRSAGAR - Ashland MA, US
Thomas RYLL - Ashland MA, US
Assignee:
Biogen MA Inc. - Cambridge MA
International Classification:
C12N 5/00
Abstract:
Methods of improving the efficiency of production of a protein product of interest in mammalian cell culture are presented. In particular, the methods result in an increase in the quantity of a protein product produced, or decreases protein product production time in a manufacturing-scale bioreactor cell culture. The disclosed methods comprise: (a) culturing the N-1 bioreactor culture to high viable cell densities; and (b) seeding the production bioreactor culture at high viable cell seeding densities.

Medium Supplements For Improved Process Performance

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US Patent:
20150353883, Dec 10, 2015
Filed:
Jan 10, 2014
Appl. No.:
14/760182
Inventors:
- Cambridge MA, US
Kyle MCELEARNEY - Medford MA, US
Terrence Michael DOBROWSKY - Cambridge MA, US
Rashmi Rohit KSHIRSAGAR - Ashland MA, US
International Classification:
C12N 5/00
C12P 21/00
C07K 14/755
Abstract:
The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

Methods Of Preventing And Removing Trisulfide Bonds

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US Patent:
20150274808, Oct 1, 2015
Filed:
Mar 4, 2015
Appl. No.:
14/638215
Inventors:
- Cambridge MA, US
R. Blake Pepinsky - Arlington MA, US
Dingyi Wen - Waltham MA, US
Rashmi Rohit Kshirsagar - Ashland MA, US
Karin Lucas - Encinitas CA, US
International Classification:
C07K 16/00
Abstract:
The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
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Rashmi Rohit Kshirsagar from Ashland, MA, age ~48 Get Report