Ramakrishna S Madabhushi

6355709, Mar 12, 2002

Oct 15, 1997

08/950926

Ramakrishna S. Madabhushi - Foster City CA
Steven M. Menchen - Fremont CA
J. William Efcavitch - San Mateo CA
Paul D. Grossman - Burlingame CA

The Perkin-Elmer Corporation - Foster City CA

C08K 516

524104, 524210, 524211, 524216, 524555

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20Â C. to about 50Â C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5Ã10 to about 1Ã10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

6358385, Mar 19, 2002

Jan 11, 1999

09/228991

Ramakrishna S. Madabhushi - Foster City CA
Steven M. Menchen - Fremont CA
J. William Efcavitch - San Mateo CA
Paul D. Grossman - Burlingame CA

The Perkin-Elmer Corporation - Foster City CA

B01D 5702

204451, 204450, 204453, 204601, 204603, 204454, 204452

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20Â C. to about 50Â C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5Ã10 to about 1Ã10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

6646084, Nov 11, 2003

Mar 7, 2002

10/094397

Ramakrishna S. Madabhushi - Fremont CA
Stuart A. Gammon - Tracy CA

The Regents of the University of California - Oakland CA

C08F12054

5263031, 526217, 526225

A polyacryalmide medium for the electrophoretic separation of biomolecules. The polyacryalmide medium comprises high molecular weight polyacrylamides (PAAm) having a viscosity average molecular weight (M ) of about 675-725 kDa were synthesized by conventional red-ox polymerization technique. Using this separation medium, capillary electrophoresis of BigDye DNA sequencing standard was performed. A single base resolution of Ë725 bases was achieved in Ë60 minute in a non-covalently coated capillary of 50 m i. d. , 40 cm effective length, and a filed of 160 V/cm at 40Â C. The resolution achieved with this formulation to separate DNA under identical conditions is much superior (725 bases vs. 625 bases) and faster (60 min. vs. 75 min. ) to the commercially available PAAm, such as supplied by Amersham.

7045048, May 16, 2006

Dec 28, 2001

10/040539

Ramakrishna S. Madabhushi - Foster City CA, US
Steven M. Menchen - Fremont CA, US
J. William Efcavitch - San Carlos CA, US
Paul D. Grossman - Hillsborough CA, US

Applera Corporation - Foster City CA

B10D 57/02

204450, 204451, 204601, 524555

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20 C. to about 50 C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×10to about 1×10daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

7863357, Jan 4, 2011

Feb 21, 2006

11/359713

Ramakrishna S. Madabhushi - Foster City CA, US
Steven M. Menchen - Fremont CA, US
J. William Efcavitch - San Carlos CA, US
Paul D. Grossman - Hillsborough CA, US

Life Technologies Corporation - Carlsbad CA

C08K 5/34

524104, 524210, 524211, 524216, 524555

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20 C. to about 50 C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×10to about 1×10daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

20060057599, Mar 16, 2006

Nov 29, 2004

11/000491

John Dzenitis - Danville CA, US
Steve Brown - Livermore CA, US
Billy Colston - San Ramon CA, US
Dora Gutierrez - Livermore CA, US
Bruce Henderer - Livermore CA, US
Benjamin Hindson - Belmont, AU
Ramakrishna Madabhushi - Fremont CA, US
Anthony Makarewicz - Livermore CA, US
Don Masquelier - Tracy CA, US
Mary McBride - Brentwood CA, US
Thomas Metz - Tracy CA, US
Shanavaz Nasarabadi - Livermore CA, US
Ujwal Setlur - Livermore CA, US
Sally Smith - Salida CA, US
Kodumudi Venkateswaran - Livermore CA, US

C12Q 1/68
C12M 1/34

435006000, 435030000, 435287200, 435288500

An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for detecting said bioagents.

20110192725, Aug 11, 2011

Dec 23, 2010

12/978219

Ramakrishna S. Madabhushi - Foster City CA, US
Steven M. Menchen - Fremont CA, US
J. William Efcavitch - San Carlos CA, US
Paul D. Grossman - Hillsborough CA, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

C25B 15/00
C25B 7/00

204454, 204451

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20 C. to about 50 C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×10to about 1×10daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

20120172692, Jul 5, 2012

Jan 5, 2011

12/985224

Janet Tamada - Stanford CA, US
Michael J. Tierney - San Jose CA, US
Ramakrishna Madabhushi - Fremont CA, US
Arvind N. Jina - San Jose CA, US

A61B 5/1486

600365

An analyte monitor having a plurality of fluid paths, each fluid path having a distal opening adapted to be disposed on one side of a stratum corneum layer of a user's skin, a proximal opening adapted to be disposed on another side of the stratum corneum layer and an interior space extending between the distal and proximal openings; a sensing zone in fluid communication with the proximal openings of the fluid paths; sensing fluid extending from the sensing zone into substantially the entire interior space of the fluid paths; and an analyte sensor adapted to detect a concentration of analyte in the sensing fluid within the sensing zone, wherein at least one of the sensing fluid and the analyte sensor comprises a catalyst for mutarotation of glucose. The invention also includes a method of using the monitor.