Inventors:
Xiaodong Wang - Dallas TX, US
Qinghua Liu - Dallas TX, US
Assignee:
Board of Regents, The University of Texas System - Austin TX
International Classification:
C12P 19/34
C12P 21/06
C12Q 1/68
C07H 21/04
US Classification:
435 912, 435 6, 435 691, 536 245
Abstract:
Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be a R2D2 protein and the Dicer protein may be a Dicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.
