Inventors:
Paul Melman - Newton MA
Shmuel Halevi - Chestnut Hill MA
Assignee:
BioTell, Inc. - Brookline MA
International Classification:
G01N 2117
US Classification:
385 12, 385 40, 356445, 422 8211
Abstract:
The invention features a waveguide based devices, methods, and systems to increase sensitivity of surface plasmon resonance (SPR) measurement through the use of differential detection. The enhanced sensitivity enables analysis and detection of a wide range of analytes including, for example, DNA, antibodies, proteins, and other chemical compounds. These methods achieve this result by sampling the SPR response curve at more than one point. This can be achieved using a detection device with sets of optical waveguides having distinct propagation parameters, or by using light of different wavelengths. These methods are suitable for multi-analyte and multi-sample applications in a miniaturized detection system. Furthermore, this invention makes use of an alternating polarity electric field to reduce nonspecific analyte binding and detection time.