Patrycja Nowakowski

Belmont, MA

Pharmaceuticals

Morphic Therapeutic Jan 2019 - Jun 2020
Associate Scientist Ii
Juno Therapeutics, Inc. Aug 2015 - Mar 2017
Associate Scientist, Biotherapeutics Discovery
Novartis Institutes For Biomedical Research (Nibr) Oct 2005 - Jul 2015
Research Associate Ii
Harvard Medical School Massachusetts General Hospital Cancer Center Nov 2002 - Sep 2005
Biotechnologist
National Institute of Cardiology Department of Clinical Biochemistry Oct 2001 - Oct 2002
Research Assistant

Drug Discovery
Molecular Biology
Antibodies
Cell Biology
Cell Culture
Life Sciences
Biotechnology
Oncology
Cell
Genotyping
Genetics
Sds Page
Polymerase Chain Reaction
Cancer

English
Polish

Radiation Safety

Novartis Institutes for BioMedical Research

Oct 2005 to 2000
Scientist I
Novartis Institutes for BioMedical Research

2005 to 2000
Research Associate II
Novartis Institutes for BioMedical Research

2005 to 2000
Research Associate I
Harvard Medical School / Massachusetts General Hospital Cancer Center
Charlestown, MA
Nov 2002 to Sep 2005
Biotechnologist
National Institute of Cardiology, Department of Clinical Biochemistry, Molecular Biology Laboratory
Warszawa, mazowieckie
Oct 2001 to Oct 2002
Research Assistant

Warsaw University
Warszawa, mazowieckie
1996 to 2001
Master of Science in Biotechnology, Genetics

Experienced in characterization and lead selection process of antibody drug conjugate (ADC) cancer therapeutics<br/> Highly proficient in culturing and testing large numbers of cell lines (up to 30 at the time) including diverse array<br/>of cell types (human, mammalian, Ba/F3 - a murine interleukin-3 dependent pro-B cell lines, fly) using various<br/>cell culture systems (2D cell culture; 3D-Spheroids, Transwell Migration chambers; suspension culture)<br/> Extensive experience in running proliferation, migration, and "piggy-back" assays (using variety of detection<br/>methods: CellTiter-Glo, BrdU, DAPI) in both 96- and 384-well format, manually and in semi-automated manner<br/> Expertise in protein isolation, purification, and analysis (SDS-PAGE; Western blots; ELISAs, 3D-gels, Immuno-<br/>percipitations, densitometry analysis, DELFIA In-Cell Westerns, Fixed Cell ELISAs, and Adhesion Assays)<br/> Excellence in molecular biology techniques (DNA cloning, including recombination-based cloning, Gateway<br/>cloning, PCR, genomic and plasmid DNA isolation, site-directed mutagenesis, DNA sequencing, labeling,<br/>Southern blotting, RNA isolation, Northern blotting, RNA interference applications)<br/> Proficient in fluorescence-activated cell sorting (FACS), pharmacokinetic and pharmacodynamic analysis<br/> Diligent, result-driven team player with excellent organizational and communication skills