Inventors:
Margit C. Ross - Johnston IA
Laura A. Church - Des Moines IA
Patrea M. Hill - Des Moines IA
William J. Gordon-Kamm - Urbandale IA
Keith S. Lowe - Johnston IA
George J. Hoerster - Des Moines IA
Dennis L. Bidney - Des Moines IA
Assignee:
Pioneer Hi-Bred International, Inc. - Des Moines IA
International Classification:
A01H 500
US Classification:
800290, 435468, 800278, 800280, 800288, 800312, 8003201, 8003202, 8003203, 536 237, 536 2372
Abstract:
The invention provides improved plant transformation methods. In particular the method provides increased transformation freequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with at least one polynucleotide of interest. The target cell has been previously transformed to stimulate growth of the cell and has gone through at least one cell division.