Inventors:
Scott Morrical - South Burlington VT, US
Na Qian - Colchester VT, US
Assignee:
University of Vermont and State Agricultural College - Burlington VT
International Classification:
C12Q 1/68
C12N 9/22
Abstract:
The invention relates to products and methods for determining, localizing, and quantitating single-stranded polynucleotides. The methods of the invention include the use of Gp32F protein and mutant Gp32F protein to monitor polynucleotide reactions such as DNA replication, annealing, and excision and to determine single-stranded polynucleotide structures such as gaps, flaps, and bubbles.