Inventors:
Moon Young Lim - Redwood City CA, US
Cynthia A. Edwards - Morgan Hill CA, US
Kirk E. Fry - Palo Alto CA, US
Thomas W. Bruice - Carlsbad CA, US
Douglas B. Starr - Mountain View CA, US
Megan E. Laurance - San Francisco CA, US
Yan Kwok - Sunnyvale CA, US
Albert W. Tam - San Francisco CA, US
Assignee:
Genelabs Technologies, Inc. - Redwood City CA
International Classification:
C12Q 1/68
C07H 21/04
US Classification:
435 6, 435 721, 435 723, 435 691, 4352351, 436501, 536 231, 536 234, 536 235, 536 236, 536 237
Abstract:
The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.