US Patent:
20170044535, Feb 16, 2017
Inventors:
- Skokie IL, US
Ashley Mae Jacobi - Tiffin IA, US
Garrett Richard Rettig - Coralville IA, US
Mollie Sue Schubert - Cedar Rapids IA, US
Mark Aaron Behlke - Coralville IA, US
Assignee:
INTEGRATED DNA TECHNOLOGIES, INC. - Coralville IA
International Classification:
C12N 15/113
Abstract:
This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.