Inventors:
Roberta L. Farrell - Danvers MA
Thomas K. Kirk - Verona WI
Ming Tien - State College PA
Assignee:
Repligen Corporation - Cambridge MA
The United States of America as represented by the Secretary of
Agriculture - Washington DC
International Classification:
C12N 902
C12N 1500
C12N 114
C12N 914
D21C 300
C12R 1645
Abstract:
Novel lignin-degrading enzymes designated rLDM. TM. 1, rLDM. TM. 2, rLDM. TM. 3, rLDM. TM. 4, rLDM. TM. 5, and rLDM. TM. 6 are isolated and purified to the essentially pure form, wherein each rLDM. TM. is substantially free of other rLDM. TM. and native proteins, from the extracellular medium of a novel mutant microbe. The novel mutant, designated SC26, produces large amounts of the rLDM. TM. , thus facilitating the isolation and purification of them. These rLDM. TM. are useful in pulping processes to degrade and/or modify lignin.