Michael C Kim

20220403445, Dec 22, 2022

Nov 8, 2021

17/521239

- San Diego CA, US
Junhua ZHAO - San Diego CA, US
Molly HE - San Diego CA, US
Samantha SNOW - San Diego CA, US
William LIGHT - Poway CA, US
Matthew KELLINGER - San Diego CA, US
Michael PREVITE - San Diego CA, US
Michael KIM - El Cajon CA, US
Hua YU - La Jolla CA, US
Marco TJIOE - San Diego CA, US
Andrew BODDICKER - San Diego CA, US

C12Q 1/6806
C12Q 1/6834
C12Q 1/6874

The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

20220403463, Dec 22, 2022

Jul 15, 2021

17/377279

- San Diego CA, US
Junhua ZHAO - San Diego CA, US
Molly HE - San Diego CA, US
Samantha SNOW - San Diego CA, US
William LIGHT - Poway CA, US
Matthew KELLINGER - San Diego CA, US
Michael PREVITE - San Diego CA, US
Michael KIM - El Cajon CA, US
Hua YU - La Jolla CA, US
Marco TJIOE - San Diego CA, US
Andrew BODDICKER - San Diego CA, US

C12Q 1/6874

The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.