Resumes
Resumes
Melissa Hasenbank Phones & Addresses
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3467 W 1St St, Ludington, MI 49431
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Free Soil, MI
Publications
Us Patents
Signal Amplification Method For Surface Plasmon Resonance-Based Chemical Detection
View pageUS Patent:
7405054, Jul 29, 2008
Filed:
Dec 13, 2005
Appl. No.:
11/301720
Inventors:
Melissa Hasenbank - Free Soil MI, US
Paul Yager - Seattle WA, US
Elain Fu - Seattle WA, US
Kjell Nelson - Seattle WA, US
Paul Yager - Seattle WA, US
Elain Fu - Seattle WA, US
Kjell Nelson - Seattle WA, US
Assignee:
University of Washington UW Tech Transfer - Invention Licensing - Seattle WA
International Classification:
G01N 33/53
G01N 33/553
G01N 33/553
US Classification:
435 79, 422 8211, 435 6, 435 792, 4352872, 4352887, 435808, 436164, 436512, 436514, 436524, 436525, 436805, 436827
Abstract:
The present invention provides methods and compositions for amplifying the detection signal in surface plasmon resonance (SPR)-based flow systems. The signal amplification methods comprise the use of well established marker systems that provide a precipitate. The marker systems include, for example, enzyme and nucleation systems. Enzymes suitable for use as a marker system include peroxidases and phosphatases. The amplification system is useful in any SPR-based detection system including microfluidic systems, e. g. , “lab on a chip” systems and the like. The methods can comprise any SPR-based assay format, including typical immunoassay formats. The immunoassay formats can include competitive and sandwich assays. Analyte capture agents can include antibodies, lectins, carbohydrates, polynucleotides, receptor proteins, and the like.