Matthew D Disney

8617819, Dec 31, 2013

Sep 19, 2005

11/230308

Timothy M. Swager - Newton MA, US
Peter H. Seeberger - Zurich, CH
Juan Zheng - Cambridge MA, US
Matthew D. Disney - Williamsville NY, US

Massachusetts Institute of Technology - Cambridge MA

G01N 33/53
G01N 21/76
G01N 33/533

435 71, 436172, 436546

The present invention generally relates to organic polymers able to participate in an analyte-recognition process, where an analyte facilitates an energy transfer between an energy donor and an energy acceptor. Certain embodiments of the invention make use of fluorescent conjugated polymers, such as poly(phenylene ethynylene)s and other polymers comprising pi-conjugated backbones. For example, one aspect of the invention provides a fluorescent conjugated polymer and an indicator that can interact with each other in the presence of an analyte to produce an emissive signal. In some cases, the interaction may include energy exchange mechanisms, such as Dexter energy transfer or the strong coupling effect. The interaction of the conjugated polymer and the indicator, in some instances, may be facilitated through specific interactions, such as a protein/carbohydrate interaction, a ligand/receptor interaction, etc. Another aspect of the invention provides for the detection of biological entities, for example, pathogenic bacteria such as , or viruses such as influenza virus.

20080188377, Aug 7, 2008

Nov 29, 2007

11/998466

Matthew D. Disney - Williamsville NY, US
Jessica L. Childs-Disney - Williamsville NY, US

C40B 30/04

506 9

Disclosed are methods for identifying a nucleic acid (e.g., RNA, DNA, etc.) motif which interacts with a ligand. The method includes providing a plurality of ligands immobilized on a support, wherein each particular ligand is immobilized at a discrete location on the support; contacting the plurality of immobilized ligands with a nucleic acid motif library under conditions effective for one or more members of the nucleic acid motif library to bind with the immobilized ligands; and identifying members of the nucleic acid motif library that are bound to a particular immobilized ligand. Also disclosed are methods for selecting, from a plurality of candidate ligands, one or more ligands that have increased likelihood of binding to a nucleic acid molecule comprising a particular nucleic acid motif, as well as methods for identifying a nucleic acid which interacts with a ligand.

20080227213, Sep 18, 2008

Feb 25, 2008

12/072291

Matthew D. Disney - Williamsville NY, US

G01N 33/00

436 94

Disclosed are RNA targeting compounds having the formula:wherein j is an integer from 1 to 100; each i is the same or different and is zero or an integer from 1 to 100; each Zrepresents the same or different linking moiety; each Ris the same or different and represents an alkyl group or an aryl group; each Qrepresents the same or different RNA binding ligand; Qis an alkyl group; Qis a halogen, an alkyl group, an aryl group, or an amine. Also disclosed are RNA targeting compounds that include a polymer backbone and two or more pendant RNA binding ligands that are bound to the polymer backbone. Methods for using the subject RNA targeting compounds to treat myotonic dystrophy and other diseases are also disclosed, as are compounds that can be used to prepare the subject RNA targeting compounds.

6958215, Oct 25, 2005

Mar 15, 2000

09/936146

Stephen M. Testa - Lexington KY, US
Matthew D. Disney - Rochester NY, US
Sergei M. Gryaznov - San Mateo CA, US
Douglas H. Turner - Pittsford NY, US

Geron Corporation - Menlo Park CA
The University of Rochester - Rochester NY

C12Q001/68
C12N015/00
C12N015/63
C12N001/20
C12N021/04

435 6, 4353201, 4352528, 435174, 435183, 382129, 382133, 382153, 382173, 382286, 382291, 702 19, 702 22, 935 10, 935 24, 935 72, 536 221

A method of inhibiting the self-splicing of a Group I intron is disclosed. The method uses an oligonucleotide having a sequence essentially identical to a guide sequence found in the 5′ flanking exon and terminates with a 3′ ribonucleoside. Usually the oligonucleotide has N3′→P5′ phosphoramidate or N3′→P5′ thiophiosphoramidate linkages rather than phosphodiester linkages. A method of inhibiting the growth of organisms having Group I intron, particularly certain pathogenic fungi including , and using the oglionucleotide is also provided.

20220267839, Aug 25, 2022

Jun 24, 2020

17/622005

- La Jolla CA, US
Matthew D. Disney - Jupiter FL, US

C12Q 1/6869
C12Q 1/6806

The invention is directed to a method of identifying the interactions of RNA such as miRNA with small molecules interacting with RNA (SMIRNAs). A candidate SMIRNA group is associated with a photoaffinity diazirene group that can form a covalent complex with an RNA target site and with an alkyne group that can be used in subsequent “click chemistry’ reactions such as a “CuAAC” reaction, a copper-catalyzed alkyne-azide cy-cloaddition to yield a stable triazole ring. By this means, the RNA binding site of the small molecule can be identified via isolation of the RNA-targetSMIRNA covalent complex and reverse transcription of the RNA followed by DNA sequencing of the reverse transcription product. Sites on the RNA blocked during reverse transcription by the covalently bound SMIRNA are identified as terminations in the sequence compared to the native RNA.

20220251545, Aug 11, 2022

Jun 25, 2020

17/622032

- La Jolla CA, US
Matthew D. Disney - Jupiter FL, US

C12N 15/10
G01N 33/543

A method is described to define the binding of fragments onto RNA targets and to use this profiling to enable the design of small molecules targeting RNA. The method comprises exposing a labeled RNA target to a small molecule fragment appended with diazirine and an alkyne moiety. Exposure of the compounds to light produce a reactive intermediate from the diazirine moiety that will react with sites in the RNA that are proximal to the small molecule fragments binding site. The RNAs that are reacted with the fragments are captured by using a biotin azide or azide-displaying beads that react with the alkyne moiety in the presence of a Cu(I) catalyst using click chemistry. Biotinylated products are captured with streptavidin resin. The amount of labeled RNA captured by the resin/beads is measured, thereby identifying which fragments bind an RNA target. The binding site of the fragment is determined by RT-PCR.

20230041228, Feb 9, 2023

Feb 16, 2018

16/486211

- Gainesville FL, US
Matthew D. Disney - Jupiter FL, US

A61K 31/496
A61P 35/00

Herein, we describe the identification of a small molecule named Targapremir-210 that binds to the Dicer site of the miR-210 hairpin precursor. This interaction inhibits production of the mature miRNA, de-represses glycerol-3-phosphate dehydrogenase 1-like enzyme (GPD1 L), a hypoxia-associated protein negatively regulated by miR-210, decreases HIF-1 a, and triggers apoptosis of triple negative breast cancer cells only under hypoxic conditions. Further, Targapremir-210 inhibits tumorigenesis in a mouse xenograft model of hypoxic triple negative breast cancer. We applied Chemical Cross-Linking and Isolation by Pull Down (Chem-CLIP) to study the cellular selectivity and the on- and off-targets of Targapremir-210. Targapremir-210 selectively recognizes the miR-210 precursor and can differentially recognize RNAs in cells that have the same target motif but have different expression levels, revealing this important feature for selectively drugging RNAs for the first time.

20210379188, Dec 9, 2021

Sep 26, 2019

17/284297

- La Jolla CA, US
Matthew D. Disney - Jupiter FL, US

A61K 47/55
C12N 15/11
A61K 31/704

A method for the precise cellular destruction of an oncogenic non-coding RNA with a RNA-binding small molecule conjugated with bleomycin A5 is described. The method affords reversal of phenotype. Bleomycin A5 was coupled to an RNA-binding molecule that selectively binds the microRNA-96 hairpin precursor (pri-miR-96). By coupling of bleomycin A5's free amine to the RNA-binding molecule, its affinity for binding to pri-miR-96 is >100-fold stronger than to DNA. The conjugate compound selectively cleaves pri-miR-96 in triple negative breast cancer (TNBC) cells. Selective cleavage of pri-miR-96 enhances expression of FOXO1 protein, a pro-apoptotic transcription factor that miR-96 silences, and triggers apoptosis in TNBC cells. No effects were observed in healthy breast epithelial cells. This method provides programmable control for targeting RNA through the selection of an RNA-binding molecule/bleomycin A5 conjugate and provides a facile method of mapping the cellular binding sites of an RNA-binding molecule.