Inventors:
Marjorie Oleksiak - Raleigh NC, US
Douglas Crawford - Key Biscayne FL, US
Assignee:
University of Missouri - Columbia MO
International Classification:
C40B 40/02
C12Q 1/68
C12N 9/22
US Classification:
435006000, 435199000, 435252300
Abstract:
Disclosed are methods of synthesizing a polynucleotide complementary to a target polynucleotide include steps of subjecting a non-thermophilic cell comprising a thermostable polymerase to a temperature effective to disrupt the cell to form a reaction mixture, wherein the reaction mixture comprises the target polynucleotide and one or more primers that hybridize to a sequence of the target polynucleotide or to a sequence flanking the polynucleotide and incubating the reaction mixture under conditions whereby the polynucleotide is synthesized. Also disclosed are cell libraries and kits in accordance with the invention.