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Lotien R Huang

from Woburn, MA
Age ~51
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Also known as:
Richard Huang Lotien, Richard Huang
Related to:
Mujen HuangChingya Huang
Connected to:
Abigail Huang, 39Alexandre Huang, 35Alexander L Huang, 66Alisa Huang, 64Allycia L Huang, 49Angelia Huang, 49Annabelle W Huang, 56Arnold Huang, 34Belle Huang, 53Bifang Huang, 55

Lotien Huang Phones & Addresses

  • Woburn, MA
  • Andover, MA
  • Chestnut Hill, MA
  • Brookline, MA
  • Highland Park, NJ
  • Princeton Junction, NJ
  • Princeton, NJ
  • Plainsboro, NJ

Publications

Us Patents

Method For Continuous Particle Separation Using Obstacle Arrays Asymmetrically Aligned To Fields

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US Patent:
7150812, Dec 19, 2006
Filed:
Oct 23, 2003
Appl. No.:
10/693091
Inventors:
Lotien Richard Huang - Princeton NJ, US
James Christopher Sturm - Princeton NJ, US
Assignee:
The Trustees of Princeton University - Princeton NJ
International Classification:
G01N 27/447
G01N 27/453
G01N 30/02
G01N 15/06
US Classification:
204451, 204601, 422 70, 422 6801, 422100
Abstract:
The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

Method And Apparatus For Generating Electric Fields And Flow Distributions For Rapidly Separating Molecules

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US Patent:
7597791, Oct 6, 2009
Filed:
May 15, 2002
Appl. No.:
10/147370
Inventors:
Lotien Richard Huang - Brookline MA, US
James Christopher Sturm - Princeton NJ, US
Robert Hamilton Austin - Princeton NJ, US
Assignee:
The Trustees of Princeton University - Princeton NJ
International Classification:
G01N 27/453
US Classification:
204600, 204450, 422100
Abstract:
A method and apparatus for generating tunable, uniform electric fields in fluidic applications for rapid separation of molecules, such as DNA, is provided. A region receives the molecules to be separated, the molecules being injected into the region by an injection channel connected thereto. Fluidic microchannels or resistor arrays connected to sides of the region inject currents into the region and produce electric fields in the region that can be oriented at any angle. The electric fields can separate the molecules according to size, and can be used to move or manipulate the molecules within the region. Further, the molecules can be separated by controlling fluid flows within the region to manipulate the molecules. One or more reservoirs can be attached to the fluidic microchannels for collecting the molecules after separation, movement, or manipulation.

Method For Continuous Particle Separation Using Obstacle Arrays Asymmetrically Aligned To Fields

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US Patent:
7988840, Aug 2, 2011
Filed:
Dec 15, 2006
Appl. No.:
11/640111
Inventors:
Lotien Richard Huang - Brookline MA, US
James Christopher Sturm - Princeton NJ, US
Robert Hamilton Austin - Princeton NJ, US
Assignee:
The Trustees of Princeton University - Princeton NJ
International Classification:
G01N 27/447
G01N 27/453
G01N 30/02
G01N 15/06
US Classification:
204451, 204601, 422503
Abstract:
The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

Devices And Methods For Enrichment And Alteration Of Cells And Other Particles

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US Patent:
8021614, Sep 20, 2011
Filed:
Jun 8, 2006
Appl. No.:
11/449149
Inventors:
Lotien Richard Huang - Brookline MA, US
Thomas A. Barber - Cambridge MA, US
Bruce L. Carvalho - Watertown MA, US
Ravi Kapur - Stoughton MA, US
Paul Vernucci - Billerica MA, US
Mehmet Toner - Wellesley MA, US
Zihua Wang - Newton MA, US
Assignee:
The General Hospital Corporation - Boston MA
GPB Scientific, LLC - Richmond VA
International Classification:
G01N 33/00
US Classification:
422 73, 436174, 436177, 422 50, 422 681
Abstract:
The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e. g. , fetal cells, present in a sample, e. g. , maternal blood and rare cell components, e. g. , fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e. g. , to extract clinical information from a cellular component, e. g. , a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e. g. , other nucleated cells) in the sample.

Method For Continuous Particle Separation Using Obstacle Arrays Asymmetrically Aligned To Fields

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US Patent:
8282799, Oct 9, 2012
Filed:
Jul 12, 2011
Appl. No.:
13/180823
Inventors:
Lotien Richard Huang - Brookline MA, US
James Christopher Sturm - Princeton NJ, US
Robert Hamilton Austin - Princeton NJ, US
Assignee:
The Trustees of Princeton University - Princeton NJ
International Classification:
G01N 27/447
G01N 27/453
G01N 30/02
G01N 15/06
US Classification:
204451, 204601, 422503
Abstract:
The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

Devices And Method For Enrichment And Alteration Of Cells And Other Particles

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US Patent:
8585971, Nov 19, 2013
Filed:
Apr 20, 2012
Appl. No.:
13/452054
Inventors:
Lotien Richard Huang - Chestnut Hill MA, US
Thomas A. Barber - Allston MA, US
Bruce L. Carvalho - Watertown MA, US
Ravi Kapur - Sharon MA, US
Paul Vernucci - Billerica MA, US
Mehmet Toner - Wellesley MA, US
Zihua Wang - Newton MA, US
Assignee:
The General Hospital Corporation - Boston MA
GPB Scientific, LLC - Richmond VA
International Classification:
G01N 33/00
US Classification:
422 73, 422 50, 422 681, 436174, 436177
Abstract:
The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e. g. , fetal cells, present in a sample, e. g. , maternal blood and rare cell components, e. g. , fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e. g. , to extract clinical information from a cellular component, e. g. , a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e. g. , other nucleated cells) in the sample.

Fractionation Of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis

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US Patent:
20020098504, Jul 25, 2002
Filed:
Dec 18, 2001
Appl. No.:
10/022189
Inventors:
Lotien Huang - Princeton NJ, US
James Sturm - Princeton NJ, US
Robert Austin - Princeton NJ, US
International Classification:
C12Q001/68
G06F019/00
G01N033/48
G01N033/50
B01D061/58
US Classification:
435/006000, 702/020000, 204/457000
Abstract:
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

Method For Loading And Unloading Macro-Molecules From Microfluidic Devices

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US Patent:
20040149568, Aug 5, 2004
Filed:
Oct 30, 2003
Appl. No.:
10/699097
Inventors:
Lotien Huang - Princeton NJ, US
James Sturm - Princeton NJ, US
International Classification:
C25D001/00
US Classification:
204/193000
Abstract:
The present invention provides an integrated microfluidic device comprising a sample chamber and a micro-fluidic channel, wherein the sample chamber contains two or more electrodes capable of generating an electric field in the sample chamber. The two or more electrodes create electric fields for sample elution, transfer, and unloading.
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Lotien R Huang from Woburn, MA, age ~51 Get Report