Inventors:
Dana C. Bookbinder - Corning NY
Leroy S. Hersh - Painted Post NY
Xinying Xie - Painted Post NY
Assignee:
Corning Incorporated - Corning NY
International Classification:
G01N 33545
Abstract:
A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0. 3 and 4. 0% atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystyrene substrate having a surface oxygen content of between 0. 3 and 4. 0% atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed.