Inventors:
Len J. Gawel - Ponca City OK
Thomas Ng - Portola Valley CA
James M. Odom - Wilmington DE
Richard C. Ebersole - Wilmington DE
Assignee:
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
G01N 3350
G01N 3353
C12Q 168
Abstract:
Sulfate reducing bacteria are detected by preparing a lysate so as to release an enzyme essential to derive energy by reduction of sulfate such as adenosine 5'-phosphosulfate reductase (APS reductase), containing the lysate with an antibody for the enzyme, and detecting the presence of the reaction product of the enzyme and the antibody. In one aspect, sulfate reducing bacteria are controlled responsive to such assay. In another aspect, a "test kit" is provided for carrying out the assay.