Inventors:
Omar S. Khalil - Libertyville IL
Thomas F. Zurek - River Forest IL
Kevin R. Genger - Chicago IL
Curtis J. Pepe - McHenry IL
Stephen M. Cotter - Fox Lake IL
Assignee:
Abbott Laboratories - Abbott Park IL
International Classification:
G01N 33543
Abstract:
Apparatus and method for performing a chemiluminescence assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.