Inventors:
Monsanto Technology LLC - St. Louis MO, US
Kevin Lee Deppermann - Saint Charles MO, US
Erik D. Dersch - Fitchburg WI, US
Jerald D. Heise - St. Louis MO, US
Angela Ranae Koestel - St. Louis MO, US
Cindy L. Ludwig - St. Louis MO, US
Brian J. Martinell - Mt. Horeb WI, US
Edward Williams - Madison WI, US
Assignee:
Monsanto Technology LLC - St. Louis MO
International Classification:
C12N 5/04
G01N 33/00
US Classification:
800278, 506 10, 435410, 435468, 435415, 435411, 435412, 435470, 4352831, 4354301
Abstract:
The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.