Resumes
Resumes
Kent Opheim Phones & Addresses
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6049 51St St, Seattle, WA 98115 (206) 524-8026
Publications
Us Patents
Competitive Immunoassay Method, Device And Test Kit
View pageUS Patent:
48031704, Feb 7, 1989
Filed:
Dec 24, 1986
Appl. No.:
6/946475
Inventors:
Thomas H. Stanton - Seattle WA
James A. Clagett - Everett WA
Deborah C. Schindele - Bothell WA
George E. Renzoni - Bothell WA
Niels H. Andersen - Seattle WA
Kent E. Opheim - Seattle WA
James A. Clagett - Everett WA
Deborah C. Schindele - Bothell WA
George E. Renzoni - Bothell WA
Niels H. Andersen - Seattle WA
Kent E. Opheim - Seattle WA
Assignee:
Ultra Diagnostics Corporation - Seattle WA
International Classification:
G01N 3353
G01N 33543
G01N 33543
US Classification:
436518
Abstract:
An immunoassay device including one or more reaction chambers, each adapted to receive and retain a volume of test fluid in fluid communication with nonoverlapping first, second, and third reagent-bearing surfaces. To the first surface is reversibly bound an analyte conjugate: analyte component conjugated to one or more components, termed ligand/marker, that serve ligand and marker functions as described herein. Analyte binding partner is immobilized on the second surface, and ligand/marker binding partner is immobilized on the third surface. The reaction chamber is preferably configured to receive and direct the test fluid sequentially past the first second, and third reagent surfaces. In use, analyte conjugate solubilized from the first surface completes with any analyte in the test fluid for analyte binding partner sites on the second surface. Excess analyte conjugate becomes sequestered on the third surface, where the marker activity is read to indicate analyte presence and concentration in the test fluid.