Inventors:
Stephen T. Isaacs - Orinda CA
Goerge D. Cimino - El Cerrito CA
Kenneth C. Metchette - Berkeley CA
John W. Tessman - Berkeley CA
International Classification:
C12Q 168
Abstract:
The invention is directed to isopsoralen compound compositions that contain a variety of substitutions which yet permit their binding to nucleic acid polymers. Reaction conditions that activate these bound isopsoralens results in covalent crosslinking to the nucleic acid polymers. The resultant complex is inhibited from being a template for the template-directed enzymatic synthesis of a complementary nucleic acid polymer. Beside the isopsoralens compositions, the invention includes their synthesis, the nucleic acid complex production and detection, and the above inhibitory method of use of the isopsoralens.