Inventors:
Tony Romeo - Decatur GA, US
Xin Wang - Atlanta GA, US
Kazushi Suzuki - Decatur GA, US
International Classification:
A61K038/00
C12N001/21
Abstract:
There is provided a method of reducing biofilm formation by biofilm-forming bacterial strains such as Salmonella, Klebsiella, or a related gamma proteobacteria. CsrA activity levels in the cell may be modulated to impact biofilm formation and glycogen metabolism. CsrA levels may be modulated by modulating the expression of csrB RNA and UvrY and BarA gene products. By increasing levels of CsrB, BarA or SdiA in a bacterial cell or increasing the rate of UvrY phosphorylation, one can increase the levels of active UvrY in the strain. There is also provided a modulator of CsrA activity in a bacteria comprising a nucleotide sequence containing the sequence element CAGGAUG.