Inventors:
Julio A. Camarero - Los Angeles CA, US
International Classification:
C40B 30/06
G01N 21/64
G01N 21/76
C12N 1/21
C12N 5/10
C12N 1/19
C12N 1/15
C12N 1/13
C12Q 1/68
C12N 15/63
US Classification:
506 10, 435 613, 4353201, 435325, 435348, 435419, 435363, 4352542, 43525411, 435354, 435353, 435350, 435349, 435366, 4352572, 43525231, 43525233, 4352523, 435351
Abstract:
This invention provides new combinatorial approaches for the biosynthesis and screening of cyclic peptides inside living cells. These novel approaches are useful for finding biologically relevant molecules, e.g., those able to inhibit the cytotoxicity of Anthrax Edema Factor. Key to this ‘living combinatorial’ approach is the use of a living cell as a micro-chemical factory for both synthesis and screening of potential inhibitors for a given molecular recognition event.