Jose Raul Rojas

20210254099, Aug 19, 2021

Apr 30, 2021

17/245437

- Tarrytown NY, US
Jose F. Rojas - Newburgh NY, US
Gregg S. Warshaw - Bayside NY, US
Chia-Jen Siao - New York NY, US

Regeneron Pharmaceuticals, Inc. - Tarrytown NY

C12N 15/85
C12N 9/22
C12N 15/10

Methods for introducing a scarless targeted genetic modification into a preexisting targeting vector are provided. The methods can use combinations of bacterial homologous recombination (BHR) and in vitro assembly to introduce such targeted genetic modifications into a preexisting targeting vector in a scarless manner.

20200318134, Oct 8, 2020

Apr 2, 2020

16/838621

- Tarrytown NY, US
Jose F. Rojas - Newburgh NY, US
Gregg S. Warshaw - Bayside NY, US
Chia-Jen Siao - New York NY, US

C12N 15/85
C12N 9/22
C12N 15/10

Methods for introducing a scarless targeted genetic modification into a preexisting targeting vector are provided. The methods can use combinations of bacterial homologous recombination (BHR) and in vitro assembly to introduce such targeted genetic modifications into a preexisting targeting vector in a scarless manner.

20200229409, Jul 23, 2020

Jan 16, 2020

16/744493

- Tarrytown NY, US
Brian Zambrowicz - Sleepy Hollow NY, US
Ka-Man Venus Lai - San Francisco CA, US
Charleen Hunt - Dumont NJ, US
Susannah Brydges - Putnam Valley NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US
Jose Rojas - Newburgh NY, US
Nicole Alessandri-Haber - Rye Brook NY, US
Robert Breese - Patterson NY, US
Susan D. Croll - Putnam Valley NY, US

A01K 67/027
C12N 15/85

This disclosure relates to an animal model of human disease. More specifically, this disclosure relates to a rodent model of mood disorders such as unipolar depression and an anxiety disorder. Disclosed herein are genetically modified rodent animals that carry a humanized G protein-coupled receptor 156 (GPR156) gene that encodes a mutant human GPR156 protein comprising Asp at an amino acid position corresponding to position 533 in a full length wild type human GPR156 protein.

20200208161, Jul 2, 2020

Mar 11, 2020

16/815194

- Tarrytown NY, US
John McWhirter - Hastings-on-Hudson NY, US
Corey Momont - White Plains NY, US
Caitlin L. Goshert - Boston MA, US
Lynn Macdonald - Harrison NY, US
Gregg S. Warshaw - Bayside NY, US
Jose F. Rojas - Newburgh NY, US
Ka-Man Venus Lai - Seattle WA, US
David M. Valenzuela - Yorktown Heights NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US

REGENERON PHARMACEUTICALS, INC. - Tarrytown NY

C12N 15/66
C12N 15/64
C12N 15/10

Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

20190194668, Jun 27, 2019

Mar 7, 2019

16/296003

- Tarrytown NY, US
John McWhirter - Tarrytown NY, US
Corey Momont - New York NY, US
Caitlin Montagna - Boston MA, US
Lynn Macdonald - Harrison NY, US
Gregg S. Warshaw - Bayside NY, US
Jose F. Rojas - Newburgh NY, US
Ka-Man Venus Lai - Tarrytown NY, US
David M. Valenzuela - Yorktown Heights NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US

REGENERON PHARMACEUTICALS, INC. - Tarrytown NY

C12N 15/66
C12N 15/64
C12N 15/10

Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

20190141966, May 16, 2019

Nov 9, 2018

16/185567

- Tarrytown NY, US
Jose F. Rojas - Newburgh NY, US
Jesper Gromada - Scarsdale NY, US

A01K 67/027
C12N 15/90

Non-human animal genomes, non-human animal cells, and non-human animals comprising a mutated Slc30a8 locus and methods of making and using such non-human animal genomes, non-human animal cells, and non-human animals are provided. The non-human animals can have increased insulin secretory capacity.

20190032156, Jan 31, 2019

Jul 31, 2018

16/050822

- Tarrytown NY, US
Charleen Hunt - Dumont NJ, US
Suzanne Hartford - Putnam Valley NY, US
Jose Rojas - Newburgh NY, US
David Frendewey - New York NY, US
Brian Zambrowicz - Sleepy Hollow NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US

C12Q 1/6897
A01K 67/027
C12N 9/38
A61K 49/00
C12N 15/11
C12N 9/22

Methods and compositions are provided for assessing CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo or ex vivo. The methods and compositions employ non-human animals comprising a CRISPR reporter such as a genomically integrated CRISPR reporter for detecting and measuring CRISPR/Cas-induced repair of a coding sequence for a catalytically inactive reporter protein through recombination with an exogenous donor nucleic acid. Methods and compositions are also provided for making and using these non-human animals.

20180002708, Jan 4, 2018

Jun 30, 2017

15/638832

- Tarrytown NY, US
John McWhirter - Tarrytown NY, US
Corey Momont - New York NY, US
Caitlin Montagna - Boston MA, US
Lynn Macdonald - Harrison NY, US
Gregg S. Warshaw - Bayside NY, US
Jose F. Rojas - Newburgh NY, US
Ka-Man Venus Lai - Tarrytown NY, US
David M. Valenzuela - Yorktown Heights NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US

Regeneron Pharmaceuticals, Inc. - Tarrytown NY

C12N 15/66
C12N 15/64
C12N 15/10

Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.