Work:
Dana Farber Cancer Institute
Jan 2013 to 2000
Research Fellow
Children's Hospital of Philadelphia
Philadelphia, PA
Apr 2009 to Dec 2011
Postdoctoral Fellow
University of Pennsylvania Perelman School of Medicine
Philadelphia, PA
Sep 2004 to Dec 2008
Graduate Student
Dana-Farber Cancer Institute, Harvard Medical School
Boston, MA
May 2002 to Jul 2004
Research Assistant
Institute of Biophysics
Xiamen, CN
Jan 1999 to Jul 1999
Research Assistant
Education:
University of Pennsylvania, Perelman School of Medicine
Philadelphia, PA
2004 to 2008
Ph.D. in Cell and Molecular Biology
Xiamen University
Xiamen, CN
1995 to 1999
B.S. in Cell Biology
Skills:
Cell and molecular biology: Tissue culture, Lentivirus generation, siRNA-mediated gene knockdown, transfection, stable cell line generation, various immuno-stainings, Chromatin- IP, FACS, luciferase reporter assays, cell proliferation MTT assay, cloning, DNA/RNA preparation Gateway plasmid cloning, PCR, Q-PCR, site-directed mutagenesis, mRNA microarray, in situ hybridization, Confocal Microscopy, etc. Protein biochemistry: small and large scale protein over-expression using E. coli, yeast and mammalian cell hosts, protein chromatographic purifications, in vitro binding, IP, Western blotting, ELISA, GTPase enzymatic assays, etc. Small animal models: basic handling skills, metabolic assays (glucose tolerance), organ isolation and culture (islets), embryonic tissue dissection, fixation and sectioning. Bioinformatics processing and analysis: ChIP-seq and expression array data analysis, DNA sequences, microscopic data, and protein structures.
