Resumes
Resumes
Pharmacy Manager
View pageWork:
Vons Pharmacy
Pharmacy Manager
Pharmacy Manager
Jian Gong Phones & Addresses
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544 Foothill Rd, Bridgewater, NJ 08807
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Trenton, NJ
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303 Vine St, Philadelphia, PA 19106 (267) 519-8673
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303 Vine St #304, Philadelphia, PA 19106 (267) 519-8673
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4764 Norstar Blvd #306, Liverpool, NY 13088
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24 Landers Rd, Stoneham, MA 02180 (781) 438-7025
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Ithaca, NY
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Boston, MA
Work
Company:
Vons pharmacy
Position:
Pharmacy manager
Publications
Us Patents
Biological Process Systems And Methods Using Microfluidic Apparatus Having An Optimized Electrowetting Surface
View pageUS Patent:
20200171501, Jun 4, 2020
Filed:
Oct 23, 2019
Appl. No.:
16/661310
Inventors:
- Emeryville CA, US
Magali Soumillon - Boston MA, US
Shao Ning Pei - Albany CA, US
Samira A. Nedungadi - Berkeley CA, US
Volker L.S. Kurz - Oakland CA, US
Jian Gong - Danville CA, US
Yara X. Mejia Gonzalez - Berkeley CA, US
Mckenzi S. Toh - Oakland CA, US
Brian A. Rabkin - Redwood City CA, US
Jason C. Briggs - Pleasanton CA, US
Magali Soumillon - Boston MA, US
Shao Ning Pei - Albany CA, US
Samira A. Nedungadi - Berkeley CA, US
Volker L.S. Kurz - Oakland CA, US
Jian Gong - Danville CA, US
Yara X. Mejia Gonzalez - Berkeley CA, US
Mckenzi S. Toh - Oakland CA, US
Brian A. Rabkin - Redwood City CA, US
Jason C. Briggs - Pleasanton CA, US
Assignee:
Berkeley Lights, Inc. - Emeryville CA
International Classification:
B01L 3/00
B01L 7/00
B03C 5/00
B03C 5/02
B01L 7/00
B03C 5/00
B03C 5/02
Abstract:
Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are systems for temperature control of the microfluidic device during nucleic acid library preparation and/or synthesis which can reduce temperature overshooting during heating and cooling steps.