Inventors:
Jeffrey D. Scharver - Durham NC
Assignee:
Burroughs Wellcome Co. - Research Triangle Park NC
International Classification:
C07C 2524
C07G 700
G01N 3354
Abstract:
A radioimmunoassay procedure has been discovered for bupropion [(. +-. )-2-t-butylamino-3-chloropropiophenone], an antidepressant compound, in biological fluids. Novel compounds of formula ##STR1## wherein either R. sub. 2 is oxygen and R. sub. 1 is (CH. sub. 2). sub. n -0-(CO). sub. m -(CH. sub. 2). sub. p -COOH where n is an integer from 0 to 5, m is 0 or 1, and p is an integer from 1 to 4; or R. sub. 1 is hydrogen and R. sub. 2 is N-O-(CH. sub. 2). sub. q -COOH where q is an integer from 1 to 3, and novel methods of making them and novel intermediates useful therein are disclosed. Novel immunogens for raising bupropion specific antisera, and novel methods of making them are disclosed comprising conjugates of the novel compounds and suitable carrier material. The drug is added to the antisera together with novel radiolabeled competitor of formula ##STR2## wherein R' is a suitable radioisotope as described above and R. sub. 3 is hydrogen, R. sub.