Jeffrey D Gawronski

University of Massachusetts Medical School

2004 to 2000
Research Specialist/ Postdoctoral Fellow
University of Connecticut
Storrs, CT
1995 to 2004
Graduate Researcher
University of Connecticut
Storrs, CT
1995 to 2002
Teaching Assistant
University of Connecticut
Storrs, CT
Guest Scientist Speaker

University of Connecticut
Storrs, CT
Jan 1995 to Jan 2004
PhD in Microbiology
University of Connecticut
Storrs, CT
Jan 1987 to Jan 1992
BS in Molecular and Cell Biology

Illumina next generation sequencing, sample preparation, and analysis -- Tn-Seq -- Bioinformatic analysis (UNIX environment; short read sequence alignment [Bowtie, SOAP, BWA] and genome viewer software {IGV] -- Galaxy, BLAST, MacVector, Mauve -- Helicos DNA sequencing and RNA-seq analysis -- Bacterial genetics -- Traditional and recombinational/Gateway cloning -- Site-directed mutagenesis -- Transposon mutagenesis -- Primer design -- Inverse PCR -- Long PCR -- Fusion PCR -- Conjugation -- Allelic exchange -- Electroporation -- Genomic DNA purification -- Library construction -- DNA sequencing -- Genetic footprinting -- Statistical analysis [Prism, R] -- Strong Excel skills -- Recombinant protein expression and purification [on exchange, affinity and gel filtration chromatography] -- Enzyme kinetics -- Phylogenetic analyses [PAUP, Phylip, MrBayes, PAML, Tree-Puzzle, ClustalX, Treeview] -- Microbial diversity analysis of 16S rRNA -- SDS-PAGE -- Western blotting -- Fluorescence microscopy [GFP, RFP, conjugated antibody] -- Mouse handling and infection models