Inventors:
Caroline Slater - Holliston MA
Jeffrey P Cambray - Lowell MA
Robert A. Obar - Walpole MA
Assignee:
Monsanto Technology, LLC - St. Louis MO
International Classification:
C07H 2104
US Classification:
536 231, 536 2431, 536 2432, 435 5, 435 6, 4352831, 4352871, 4352872, 4352887, 422 50, 422 61, 422 681, 422 8205
Abstract:
Arrays of polynucleotide or polypeptide target molecules immobilized on a surface of a substrate where the target molecules are arranged in the array according to intensity of organism expression of cognate probe molecules which hybridize to the target molecules. For instance, target molecules having a higher than average indicia of hybridization, e. g. at least a factor of 2, are segregated at a peripheral region of the substrate and at a lower surface density. Preferred arrays can contain animal, plant or microorganism target molecules including. Diagnostic arrays can comprise targets from mixed species, e. g. human, mouse and virus; plant breeding arrays can comprise targets from mixed plants, e. g. , maize, soy, cotton, wheat, rice, canola and potato.