Inventors:
Craig Eric Curless - Moorpark CA, US
Jeffrey Burke Baclaski - Thousand Oaks CA, US
Assignee:
Amgen Inc. - Thousand Oaks CA
International Classification:
C12P 21/04
Abstract:
The invention relates to the use of a phosphate glass as a phosphorous source in the media of high cell density bacterial fermentation processes in order to reduce metallophosphate precipitation reactions. The methods of the invention demonstrate a practical method for controlling levels of metallophosphate precipitation in both the nutrient media and the fermentor which also result in increased cell densities and are useful in a variety of high cell density bacterial fermentation processes.